Sequences of PCR primers, length of PCR product, optimal annealing temperature, and sequences accession number from the NCBI Entrez search system
| Accession number | Primer (5′–3′) | Product (bp) | Annealing temperature (°C) | Cycles | |
|---|---|---|---|---|---|
| PCR, polymerase chain reaction; fw, forward; rv, reverse; bp, base pair; MMP, matrix metalloproteinase, IL18R, IL18 receptor. | |||||
| GAPDH | NM_002046 | fw: GAAGGTGAAGGTCGGAGTC | 226 | 60 | 30 |
| rv: GAAGATGGTGATGGGATTTC | |||||
| MMP-1 | NM_002421 | fw: CTGTTCAGGGACAGAATGTGCT | 85 | 60 | 32 |
| rv: TCGATATGCTTCACAGTTCTAGGG | |||||
| MMP-3 | J03209 | fw: CTCACAGACCTGACTCGGTT | 294 | 58 | 30 |
| rv: CACGCCTGAAGGAAGAGATG | |||||
| MMP-13 | NM_002427 | fw: TCCTCTTCTTGAGCTGGACTCATT | 97 | 60 | 32 |
| rv: CGCTCTGCAAACTGGAGGTC | |||||
| Aggrecanase-1 | AF148213 | fw: CCACGGGTGGTAGATG | 197 | 60 | 35 |
| rv: TTGTTGTATCCGTACCTG | |||||
| Aggrecanase-2 | AF142099 | fw: CAGAAACAACGGACGC | 293 | 60 | 35 |
| rv: CGGAATTACTGTACGGC | |||||
| IL18 | E15641 | fw: GCTTGAATCTAAATTATCAGT | 342 | 56 | 35 |
| rv: GAAGATTCAAATTGCATCTTAT | |||||
| IL18Rα | BD130236 | fw: GTCAACAGCACATCATTGTAT | 670 | 60 | 38 |
| rv: TAG AATTCTTATGTTTTTCCATCT | |||||
| IL18Rβ | AF077346 | fw: TACCAGAGCAAGGATCAGACGC | 772 | 56 | 25 |
| rv: CAATCCCATTCCATTGTCCATC | |||||
| Nested rv: CCAGGGCTCATTTCACCATTC | 630 | 56 | 20 | ||