Primary and secondary antibodies used for immunofluorescence double staining
| Clone | Specificity | Host | Source |
|---|---|---|---|
| Primary antibodies (unconjugated) | |||
| 4KB5 | CD45RA | Mouse | Dako, Glostrup, Denmark |
| PD7/26 | CD45RB | Mouse | Dako |
| OPD4 | CD45RO | Mouse | Dako |
| UCHT-1 | CD3 | Mouse | BD |
| Primary antibodies (conjugated) | |||
| UCHT-1 | CD3-FITC | Mouse | BD Pharmingen, San Diego, USA |
| M-T271 | CD27-FITC | Mouse | BD |
| Secondary antibodies | |||
| R0270 rabbit antimouse TRITC | Mouse IgG | Rabbit | Dako |