Diagnostic performance of commercially available enzyme linked immunosorbent assay kits for detection of PR3-ANCA compared with direct immunofluorescence using optimum cut off levels calculated by ROC curve analysis
| Test | Optimum cut off | Sensitivity | Specificity | PPV | NPV | AUC | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | 95% CI | Mean | 95% CI | Mean | 95% CI | |||||||||||||
| AUC, area under the curve; CI, confidence interval; IFT, indirect immunofluorescence technique; IHELI, in-house enzyme linked immunosorbent assay; NPV, negative predictive value; PPV, positive predictive value; PR3-ANCA, antineutrophil cytoplasmic antibodies to proteinase 3; ROC, receiver operating characteristic. | ||||||||||||||||||
| IFT (CA) | >0 | 73.3 | 44.9 to 92.0 | 98 | 91.7 to 99.7 | 84.62 | 96.15 | 0.863 | 0.76 to 0.92 | |||||||||
| IHELI | >2.55 | 66.7 | 38.4 to 88.1 | 100 | 91.7 to 99.7 | 24.56 | 93.75 | 0.821 | 0.72 to 0.89 | |||||||||
| A | >0.53 | 80.0 | 51.9 to 95.4 | 90 | 79.1 to 95.5 | 70.59 | 100 | 0.900 | 0.81 to 0.96 | |||||||||
| B | >3.46 | 66.7 | 38.4 to 95.4 | 96 | 89.3 to 99.5 | 83.33 | 94.34 | 0.749 | 0.64 to 0.84 | |||||||||
| E | >1 | 66.7 | 38.4 to 88.1 | 96 | 85.0 to 98.3 | 83.33 | 94.34 | 0.791 | 0.69 to 0.87 | |||||||||
| F | >3.27 | 60.0 | 32.3 to 83.6 | 90 | 81.0 to 96.5 | 50.0 | 87.23 | 0.724 | 0.61 to 0.82 | |||||||||
| G | >1.29 | 66.7 | 38.4 to 88.1 | 96 | 91.7 to 99.7 | 100 | 89.29 | 0.820 | 0.72 to 0.89 | |||||||||
| H | >0.64 | 66.7 | 38.4 to 88.1 | 94 | 89.3 to 99.5 | 90.90 | 92.59 | 0.834 | 0.73 to 0.91 | |||||||||
| I | >2.74 | 86.7 | 59.5 to 98.0 | 94 | 82.9 to 97.4 | 72.72 | 100 | 0.900 | 0.81 to 0.96 | |||||||||
| J | >0.64 | 73.3 | 44.9 to 92.0 | 90 | 81.0 to 96.5 | 70.58 | 93.61 | 0.896 | 0.81 to 0.96 | |||||||||
| K | >11.29 | 66.7 | 38.4 to 88.1 | 100 | 91.7 to 99.7 | 100 | 90.90 | 0.875 | 0.78 to 0.93 | |||||||||
| L | >0 | 53.3 | 26.6 to 78.7 | 100 | 91.7 to 99.7 | 100 | 87.72 | 0.756 | 0.65 to 0.84 | |||||||||
| M | >5.77 | 53.3 | 26.6 to 78.7 | 92 | 85.0 to 98.3 | 72.72 | 92.59 | 0.729 | 0.62 to 0.82 | |||||||||