Diagnostic performance of commercially available enzyme linked immunosorbent assay kits for detection of MPO-ANCA compared with direct immunofluorescence using cut off levels provided by the manufacturer
| Test | Provided cut off | Sensitivity | Specificity | |||
|---|---|---|---|---|---|---|
| IFT, indirect immunofluorescence technique; IHELI, in-house enzyme linked immunosorbent assay; MPO-ANCA, antineutrophil cytoplasmic antibodies to myeloperoxidase; neg, negative; PA, P-ANCA; pos, positive. | ||||||
| IFT (PA) | 1:16 | 86.7 | 98 | |||
| IHELI | >20 | 60.0 | 100 | |||
| A | >6 | 26.7 | 100 | |||
| B | <10 neg, >15 pos | 66.7 | 98 | |||
| E | <7 neg, >10 pos | 60.0 | 100 | |||
| F | >15 | 53.3 | 100 | |||
| G | >9 | 46.7 | 100 | |||
| H | <0.9 neg, >1.1 pos | 66.7 | 100 | |||
| I | >20 | 46.7 | 100 | |||
| J | >6 | 53.3 | 100 | |||
| K | <20 neg, >30 pos | 46.7 | 100 | |||
| L | <20 neg, >25 pos | 60.0 | 100 | |||
| M | >15 | 53.3 | 100 | |||