Diagnostic performance of commercially available enzyme linked immunosorbent assay kits for detection of PR3-ANCA compared with direct immunofluorescence using cut off levels provided by the manufacturer
| Test | Provided cut off | Sensitivity | Specificity | |||
|---|---|---|---|---|---|---|
| CA, C-ANCA; IFT, indirect immunofluorescence technique; IHELI, in-house enzyme linked immunosorbent assay; neg, negative; pos, positive; PR3-ANCA, antineutrophil cytoplasmic antibodies to proteinase 3. | ||||||
| IFT (CA) | 1:16 | 73.3 | 98 | |||
| IHELI | <2.5 neg, >5 pos | 66.7 | 100 | |||
| A | >2.0 | 60.0 | 100 | |||
| B | <10 neg, >15 pos | 13.3 | 100 | |||
| E | <7 neg, >10 pos | 46.7 | 100 | |||
| F | >15 | 26.7 | 98 | |||
| G | >3.5 | 60.0 | 100 | |||
| H | <0.9 neg, >1.1 pos | 60.0 | 98 | |||
| I | >20 | 60.0 | 100 | |||
| J | >2 | 60.0 | 100 | |||
| K | <20 neg, <30 pos | 60.0 | 100 | |||
| L | <10 neg, >20 pos | 40.0 | 100 | |||
| M | >15 | 26.7 | 96 | |||