Antiproliferative effects of DMARDs and other drugs against CEM (T) and CEM/SSZ cells
| DMARD/drug | IC50†[RF]‡ | ||
|---|---|---|---|
| CEM (T) | CEM/SSZ1.5 | CEM/SSZ2.5 | |
| Statistical significance (analysis of variance test): IC50 CEM/SSZ v IC50 CEM (T): *p<0.05; **p<0.01; ***p<0.001; NS, not significant. | |||
| †IC50 values were determined after 72 hours of DMARD/drug exposure. Values are mean (SD) of 3–6 separate experiments; ‡(RF), resistance factor: IC50 CEM/SSZ:IC50 CEM (T); §100 nM Ko143. | |||
| ND, not determined. | |||
| SSZ (µM) | 400 (30) [1] | 1720 (170) [4.3]*** | 2550 (212) [6.4]*** |
| Mitoxantrone (nM) | 0.47 (0.08) [1] | 1.94 (0.67) [4.1]** | 3.3 (0.6) [7.0]*** |
| Mitoxantrone (+Ko143)§ | 0.33 (0.07) [1] | 0.36 (0.06) [1.1] | ND |
| Leflunomide (µM) | 17.2 (1.9) [1] | 37.4 (14.4( [2.2]NS | 88.0 (37.4) [5.1]** |
| Methotrexate (nM) | 8.4 (0.7) [1] | 9.0 (0.7) [1.1]NS | 15.1 (5.1) [1.8]* |
| MG132 (nM) | 39.5 (5.0) [1] | 68.1 (8.8) [1.7]*** | 63.1 (7.9) [1.6]** |
| Chloroquine (µM) | 44.5 (7.5) [1] | 37.8 (8.7) [0.8]NS | 23.3 (13.9) [0.5]* |
| Cyclosporin A (µM) | 5.7 (0.8) [1] | 3.6 (0.8) [0.6]** | 4.0 (0.2) [0.7]* |
| Dexamethasone (nM) | 41.5 (13.1) [1] | 3.4 (0.6) [0.08]** | 6.4 (0.8) [0.15]** |