Table 1

Primers and reaction mix composition for Fas promoter genotyping by haplotype specific PCR

PrimerSpecificityPosition in Fas reference sequence*Primer sequence (5′ to 3′)
2092−670G950–967GTGCACAAGGCTGGCACG
2093−670G950–967GTGCACAAGGCTGGCACA
2096−1377G1693–1674TGAGAGGCTCACAGACGTTC
2097−1377A1693–1674TGAGAGGCTCACAGACGTTT
210Control†ATGATGTTGACCTTTCCAGGG
211Control†TTCTGTAACTTTTCATCAGTTGC
Primer mixHaplotype specificitySense primerAntisense primer
*Genbank accession number X87625; †Control primers amplify a conserved region of the adenomatous polyposis coli gene.
1238−1377G, −670G20922096
1239−1377A, −670G20932096
1240−1377G, −670A20922097
1241−1377A, −670A20932097