Sequences of PCR primers for MMPs
| Primers (5′–3′) | |||
|---|---|---|---|
| Sense | Antisense | Expected product size bp | |
| PCR, polymerase chain reaction; MMP, matrix metalloproteinase; G3PDH, glyceraldehyde-3-phosphate dehydrogenase. | |||
| MMP-1 | GGGAACCCTCGCTGGGAGCAAA | GGCCGAGTTCATGAGCCGCAAC | 352 |
| MMP-2 | CAGCCTGGGACTGCCCCCTGAT | CAGGCCCCTCCGGGTCCTTCTC | 400 |
| MMP-3 | CCTGGGACGCCAGCCAACTGTG | TGCTTGGGAAAGCCTGGCTCCAT | 425 |
| MMP-7 | CCTCAGGAGGCGGGAGGCATGA | GCTCCTCGCGCAAAGCCAATCA | 425 |
| MMP-8 | GCCTCGCTGTGGAGTGCCTGACA | GCGAGCCCCAAAGAATGGCCAAA | 414 |
| MMP-9 | GCCATCGCGGAGATTGGGAACC | CGGGGAACATCCGGTCCACCTC | 400 |
| MMP-10 | ATGCGCAAGCCCAGGTGTGGAG | GCCCCAGGGAGTGGCCAAGTTC | 418 |
| MMP-11 | CCCTGGCCCACTGTCACCTCCA | GGCAGCATGGACCGGGAACCTC | 393 |
| MMP-12 | TGAAAGTGACCGGGCAACTGGACA | TGTGCATCCCCTCCAATGCCAGA | 370 |
| MMP-13 | GCGGGGTTCCTGATGTGGGTGA | GGCCGAACTCATGCGCAGCAAC | 390 |
| MMP-14 | ATTCGCAAGGCGTTCCGCGTGT | CGGTCATCATCGGGCAGCACAA | 398 |
| MMP-15 | CAATGAGCGCCTGCGGATGGAG | CGTTCACCGTCCGTGCCACCTC | 263 |
| MMP-16 | GACAGTGCCCCCACACCGCTCT | TAGGAGGCAAGCCCCGCCAGAA | 260 |
| MMP-17 | GTGCGCGTCTGGCAGCTGTACG | AGACGGGGCGCGGGTATCCTTC | 403 |
| G3PDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | 450 |