Table 1

Comparison of culture, DNA sequence analysis, and clinical diagnosis

Patient Sample Culture PCR/sequence analysis % Identity/length of overlap (bp) Diagnosis/comment
1Cells Staphylococcus aureus Staphylococcussp.100/301Purulent arthritis, prosthetic joint, rheumatiod arthritis, sepsis
2Cells Escherichia coli Enterobactersp.1-150 100/359Purulent arthritis, diabetes mellitus, joint operation, sepsis
3Swab Staphylococcus aureus Staphylococcussp.100/360Purulent arthritis, sepsis
4Swab Streptococcus gordonii Streptococcus sanguis 99.45/361Purulent arthritis, prosthetic joint, rheumatoid arthritis
5Cells Viridans streptococcus Streptococcus gordonii 99.65/287Purulent arthritis, intra-articular injection
6Swab Streptobacillus moniliformis Streptobacillus moniliformis 99.45/359Purulent arthritis, two month history of infection
7Swab Staphylococcus epidermidis Purulent arthritis, prosthetic joint
8Cells Staphylococcus epidermidis 1-151 Purulent arthritis, prosthetic joint
9Swab Aeromonas hydrophila Purulent arthritis, gout
10Cells Escherichia coli Trauma, culture contamination
11Cells 1-152 Possible purulent arthritis, prosthetic joint
12Cells 1-152 Possible purulent arthritis, prosthetic joint
13Swab Staphylococcussp.100/259Possible purulent arthritis, unclear
14Cells Staphylococcussp.95.50/191Reactive arthritis triggered by salmonella
15Cells 1-152 Trauma
16Swab Bacillussp.97.80/350Stress injury
17CellsChloroplast 16S rDNA1-154 97.24/282Chronic osteomyelitis
18Cells Staphylococcus aureus Staphylococcussp.99.72/362Not known
19Swab Staphylococcus aureus Staphylococcussp.99.73/363Not known
20Cells Staphylococcus aureus Staphylococcussp.99.69/326Not known
21Swab Staphylococcus aureus Staphylococcussp.100/360Not known
22Swab Staphylococcus epidermidis Not known
23Swab Staphylococcus aureus Not known
24Swab Staphylococcus aureus Not known
25Swab Acinetobacter junii 100/362Not known
  • 1-150 Several enterobacterial species (Echerichia coli, Shigella boydii, Shigella dysenteriae, Shigella flexneri, Shigella sonnei).

  • 1-151 Bacterial culture from synovial fluid was negative butStaph epidermidis was cultured from a synovial biopsy specimen. Bacterial PCR was not performed from the biopsy specimen.

  • 1-152 Based on overlapping sequences, it is possible that DNA from more than one bacterial species may exist in the sample.

  • 1-154 Several different chloroplast 16S rDNAs.