Table 4

Confirmation of anti-SSA reactivity detected by antinuclear antibody line immunoassay

Serum	A	B	C	D	E	F	G	H	Confirmation
1	N	ND	ND	ND	P	ND	ND	ND	P
2	N	N	N	P	ND	ND	ND	ND	P
3	N	N	N	N	N	N	N	N	N
4	P	P	N	P	ND	ND	ND	ND	P
5	P	N	N	N	P	ND	ND	ND	P
6	N	N	N	N	P	P	P	P	P
7	P	N	P	P	ND	ND	ND	ND	P
8	N	N	N	N	P	P	P	ND	P
9	P	P	P	N	ND	ND	ND	ND	P
10	P	N	N	P	ND	ND	ND	ND	P
11	N	N	N	N	N	P	P	P	P
12	P	ND	ND	ND	ND	ND	ND	ND	P
13	P	P	P	P	ND	ND	ND	ND	P
14	N	N	N	N	P	P	P	P	P
15	N	N	N	P	ND	ND	ND	ND	P
16	P	N	N	P	ND	ND	ND	ND	P
17	N	N	N	ND	P	P	P	P	P
18	N	N	N	P	ND	ND	ND	ND	P
19	P	P	N	P	ND	ND	ND	ND	P
20	P	N	P	P	ND	ND	ND	ND	P
21	N	N	N	N	P	P	P	P	P
22	N	N	N	P	ND	ND	ND	ND	P
23	P	P	P	ND	ND	ND	ND	ND	P
24	N	N	N	N	P	N	N	P	P
25	N	N	N	N	N	N	N	N	N
26	P	P	P	ND	ND	ND	ND	ND	P
27	N	N	N	P	ND	ND	ND	ND	P
28	P	P	?	P	ND	ND	ND	ND	P
29	P	P	P	P	ND	ND	ND	ND	P
30	P	P	N	ND	ND	ND	ND	ND	P
31	N	N	N	N	P	N	P	N	P
32	P	P	P	P	ND	ND	ND	ND	P
33	P	P	ND	P	ND	ND	ND	ND	P
34	N	N	N	N	P	N	N	P	P
35	N	N	N	N	P	P	P	P	P
36	N	N	N	N	N	ND	ND	ND	N
37	P	P	P	P	ND	ND	ND	ND	P
38	N	N	N	N	N	N	N	N	N
39	P	P	P	P	ND	ND	ND	ND	P
40	N	N	N	N	N	N	N	N	N
41	N	N	N	N	N	N	N	N	N
42	N	P	N	ND	ND	ND	ND	ND	P
43	P	P	P	P	ND	ND	ND	ND	P
44	P	P	P	ND	ND	ND	ND	ND	P
45	P	P	P	P	ND	ND	ND	ND	P
46	P	P	P	ND	ND	ND	ND	ND	P
47	N	N	N	ND	N	N	N	N	N
48	N	N	N	ND	N	N	P	P	P
49	N	N	N	ND	N	N	N	N	N
50	P	ND	ND	ND	ND	ND	ND	ND	P
51	N	ND	ND	ND	N	N	N	P	P
52	N	N	N	ND	N	N	N	N	N
53	N	ND	ND	ND	P	ND	ND	ND	P
54	P	ND	ND	ND	ND	ND	ND	ND	P
55	N	N	N	ND	P	N	N	P	P
56	P	ND	ND	ND	ND	ND	ND	ND	P
57	P	ND	ND	ND	ND	ND	ND	ND	P
58	N	N	N	ND	P	N	N	N	P
59	P	ND	ND	ND	ND	ND	ND	ND	P
60	P	ND	ND	ND	ND	ND	ND	ND	P
61	N	N	N	ND	P	P	P	P	P
62	N	N	N	ND	N	N	N	N	N
63	N	ND	ND	ND	N	ND	ND	ND	N
64	P	ND	ND	ND	ND	ND	ND	ND	P
65	N	N	N	ND	N	P	P	P	P

N = negative; P = positive; ND = not done.
A = Line immunoassay coated with natural SSA (Immunovision).
B = Double immunodiffusion with natural SSA (Immunovision).
C = SSA-specific staining pattern on HEp-2000 cells.
D = Immunoblot with natural SSA (Immunovision) and recombinant Ro60 (immunoreactivity with at least one of these proteins is considered a positive result).
E = Immunoblot with HeLa S100 extract.
F = ELISA with equal amounts of baculovirus-expressed recombinant Ro52 and Ro60.
G = ELISA with baculovirus-expressed recombinant Ro52 under non-reducing conditions.
H = ELISA with baculovirus-expressed recombinant Ro52 under reducing conditions.8