Table 1

Summary of polymerase chain reaction (PCR) conditions for IL1α, IL1β, and IL1 receptor antagonist polymorphisms

	IL1α	IL1β	IL1 receptor antagonist
Primers		5CTCAGCAACACTCCTAT3	5TGGCATTGATCTGGTTCATC3	5AGGCAATAGGTTTTGAGGGCCAT3
Primers		5TCCTGGTCTGCAGGTAA3	5GTTTAGGAATCTTCCCACTT3	5TCCTCCCTGCTCCGATTCCG3

PCR conditions		94°C 1 min	95°C 2 min	96°C 1 min
		(94°C 1 min)	(95°C 1 min)	(94°C 1min)
		(46°C 1 min) × 40	(53°C 1 min) × 35	(60°C 1 min) × 30
		(72°C 1 min)	(74°C 1 min)	(70°C 1 min)
		72°C 5 min	74°C 5 min	72°C 5 min

PCR product size (after digestion)		Allele 1 = 83bp + 16bp	Allele 1 = 190bp + 114bp	Allele 1 = 410bp
		Allele 2 = 99bp	Allele 2 = 304 bp	Allele 2 = 240bp
				Allele 3 = 500bp
				Allele 4 = 325bp
				Allele 5 = 595bp