Table 3

Number of clonally accumulated T cells detected in multiple joints A  Number of clonally accumulated T cells detected in multiple lesions of five RA patients

Patients jointsTCR BV families
12345S15S26789111213S113S21415
RA1
 PBLSSSSSSSSSSSSSSSS
 left ankle4/61/32/44/5-/04/5-/7-/85/5-/10-/14-/4-/30/35/5-/6
 right ankle4/61/72/24/8-/64/5-/10-/65/8-/13-/10-/3-/30/65/11-/4
RA2
 PBL2/5SSSSS1/3S1/22/22/20/11/30/6SS
 right MP2/144/94/43/92/113/111/93/74/86/82/4S3/71/32/21/2
 right elbow2/104/94/53/72/83/121/63/74/66/62/3S3/71/62/31/6
RA3
 PBLSSSSSSSSSSSSSSSS
 right MP7/11S2/43/61/34/90/3S4/92/32/7S1/71/73/81/11
 right elbow7/130/42/23/51/54/50/90/44/82/52/30/21/41/43/61/8
RA4
 PBL3/5SSSS1/2SS1/2S3/5S0/30/41/1S
 left knee5/104/41/20/20/12/31/45/85/144/137/115/110/9S3/84/13
 right wrist5/104/61/90/20/32/21/35/65/84/147/115/10SS3/94/15
RA5
 PBLSSSSSSSSSSSSSSSS
 left knee1/36/155/60/34/43/85/84/62/73/60/30/72/70/52/40/3
 right knee1/96/65/8S4/103/85/124/102/43/60/20/72/6S2/5S
Total number in 22
BV families
Total number in
compared BV families
Average in one
BV family
16171820(average)(average)(%)
SSSSall Sall S
-/63/46/113/511437/43* 3.4/4.7
-/33/46/103/412937/61* (71)
(122)(37/52)*
S0/1S1/1279/27
5/70/1S1/712347/1232.4/6.2
5/70/10/101/612547/125(38)
(124)(47/124)
SSSSall Sall S
4/73/41/71/911540/1152.0/5.6
4/63/41/41/510640/106(36)
(111)(40/111)
SSSS229/22
1/81/21/30/613249/1322.5/6.4
1/91/21/4S12349/123(38)
(128)(49/128)
SSSSall Sall S
0/2S2/31/210238/1021.9/5.0
S0/12/11/29738/97(38)
(100)(38/100)
(117) (44)§
T cell clonality in two joints of each patient was analysed by SSCP. Numbers of SSCP bands that were detected in each joint (total clonotypes) and that were detected in both joints (common clonotypes) are shown as common clonotypes/total clonotypes. Analysis of BV10 and BV19 was not included as these two genes were recently reported to be transcribed pseudogenes.40 S, smear-like results (no band).
Averages were calculated only from the results of joints. -, In the case of RA1, clonotypes in BV5S1, 6, 7, 9, 11, 12, 13S1, 15 and 16 were not compared between the joints because of the limited amounts of the samples, and thus were omitted from the analysis(*). Average number of the total clonotypes in one joint in the five patients.§Average frequency of the common clonotypes in the five patients.
B Number of clonally accumulated T cells detected in multiple joints of RA1
Patients TCR BV families Total number in compared 11 BV families (average) Average in one BV family (%)
1 2 3 4 5S2 8 13S2 14 17 18 20
Left ankle4/63/31/43/55/54/50/31/50/45/112/528/56
Right ankle4/63/71/23/85/54/80/61/110/45/102/428/71
Left PIP4/63/71/23/65/64/80/21/80/45/122/428/652.5/5.5
Left elbow4/43/31/43/55/64/60/41/1S5/122/428/49(45)
(28/60)
  • T cell clonality in four joints of RA1 was analysed by SSCP. Comparison of the other BV families was not available because of the limited amounts of the samples. Number of SSCP bands that were detected in all four joints were counted as common clonotypes. Results are shown as common clonotypes/total clonotypes. S, smear-like results (no band).