TY - JOUR T1 - Response to: ‘Semi-quantitative analysis of line blot assay for myositis-specific and myositis-associated antibodies: a better performance?’ by Cavazzana <em>et al</em> JF - Annals of the Rheumatic Diseases JO - Ann Rheum Dis SP - e153 LP - e153 DO - 10.1136/annrheumdis-2019-215967 VL - 79 IS - 11 AU - Johan Rönnelid AU - Fabricio Espinosa-Ortega AU - Ingrid E Lundberg Y1 - 2020/11/01 UR - http://ard.bmj.com/content/79/11/e153.abstract N2 - We have with great interest read the response from Cavazzana et al.1 We agree that increasing the cut-off of the line blot test for myositis-specific and myositis-associated autoantibodies could help to avoid false-positive responses. Our experience from more than a decade of using different versions of the Euroline (Euroimmun, Lübeck, Germany) line immunoassays (LIA) is, in agreement with the suggestion from Cavazzana et al that in the absolute majority of sera with multiple autoantibody specificities the autoantibody reactivities are present in the low range. When investigating only moderate and high reactivities from our previous comparison between LIA and an immunoprecipitation-based algorithm,2 we do, however, not generally improve the kappa statistics, in the way described by Cavazzana et al. We obtained an overall agreement between both approaches of 78% (vs previous 76%) and a kappa coefficient 0.48 (vs previous 0.54). Regarding individual specificities, we did not find … ER -