TY - JOUR T1 - Regarding micro-RNA-181a-5p antisense oligonucleotides attenuate osteoarthritis in facet and knee joints JF - Annals of the Rheumatic Diseases JO - Ann Rheum Dis SP - e69 LP - e69 DO - 10.1136/annrheumdis-2019-215454 VL - 79 IS - 6 AU - Michael R Liebling Y1 - 2020/06/01 UR - http://ard.bmj.com/content/79/6/e69.abstract N2 - I read with considerable interest the paper by Nakamura and colleagues dealing with the potential therapeutic use of a locked nucleic acid, antisense oligonucleotide (LNA ASO) against a micro-RNA in osteoarthritis (OA).1 I was particularly impressed with the Osteoarthritis Research Society International and cellularity scores as well as the data on degenerative markers and chondrocyte apoptosis/death from the in vivo animal models. Nonetheless, I had a couple of questions that I hope the authors can help me with.First, was the reason you had to employ TransFectin Lipid Reagent to transfect … ER -