RT Journal Article
SR Electronic
T1 Potential involvement of OX40 in the regulation of autoantibody sialylation in arthritis
JF Annals of the Rheumatic Diseases
JO Ann Rheum Dis
FD BMJ Publishing Group Ltd and European League Against Rheumatism
SP 1488
OP 1496
DO 10.1136/annrheumdis-2019-215195
VO 78
IS 11
A1 Izumi Kurata
A1 Isao Matsumoto
A1 Ayako Ohyama
A1 Atsumu Osada
A1 Hiroshi Ebe
A1 Hoshimi Kawaguchi
A1 Shunta Kaneko
A1 Yuya Kondo
A1 Hiroto Tsuboi
A1 Azusa Tomioka
A1 Hiroyuki Kaji
A1 Takayuki Sumida
YR 2019
UL http://ard.bmj.com/content/78/11/1488.abstract
AB Objective An increased proportion of circulating follicular helper T (Tfh) cells was reported in rheumatoid arthritis (RA), but it remains uncertain how Tfh cells affect antibody hyposialylation. We investigated the regulation of autoantibody hyposialylation by Tfh cells in RA using murine model.Methods Behaviours of Tfh cells and their function on B cell promotion were analysed. Change of arthritogenicity and sialylation of autoantibodies during the course of arthritis was examined by mass spectrometry. Tfh-mediated regulation of hyposialylation was investigated, and the responsible cell surface molecule was specified both in vitro and in vivo. The relation between circulating Tfh cells and hyposialylation was analysed in patients with RA.Results An increase in Tfh, particularly interleukin-17 producing Tfh (Tfh17) cells, at the onset of arthritis and their enhancement of autoantibody production were found. Autoantibodies at the onset phase demonstrated stronger inflammatory properties than those at the resolution phase, and mass spectrometric analysis revealed their difference in sialylation. In vitro coculture showed enhanced hyposialylation by the Tfh cells via OX40, which was highly expressed in the Tfh and Tfh17 cells. Blockade of OX40 prevented the development of arthritis with reduction in Tfh17 cells and recovery of autoantibody sialylation. Analysis of patients with RA showed abundance of OX40-overexpressing Tfh17 cells, and their proportion correlated negatively with the expression of α2,6-sialyltransferase 1, an enzyme responsible for sialylation.Conclusions OX40 expressed on Tfh cells can regulate autoantibody sialylation and play a crucial role in the development of autoimmune arthritis.