RT Journal Article
SR Electronic
T1 Metabolic pathways and immunometabolism in rare kidney diseases
JF Annals of the Rheumatic Diseases
JO Ann Rheum Dis
FD BMJ Publishing Group Ltd and European League Against Rheumatism
SP 1226
OP 1233
DO 10.1136/annrheumdis-2017-212935
VO 77
IS 8
A1 Peter C Grayson
A1 Sean Eddy
A1 Jaclyn N Taroni
A1 Yaíma L Lightfoot
A1 Laura Mariani
A1 Hemang Parikh
A1 Maja T Lindenmeyer
A1 Wenjun Ju
A1 Casey S Greene
A1 Brad Godfrey
A1 Clemens D Cohen
A1 Jeffrey Krischer
A1 Matthias Kretzler
A1 Peter A Merkel
A1 ,
YR 2018
UL http://ard.bmj.com/content/77/8/1226.abstract
AB Objectives To characterise renal tissue metabolic pathway gene expression in different forms of glomerulonephritis.Methods Patients with nephrotic syndrome (NS), antineutrophil cytoplasmic antibody-associated vasculitis (AAV), systemic lupus erythematosus (SLE) and healthy living donors (LD) were studied. Clinically indicated renal biopsies were obtained at time of diagnosis and microdissected into glomerular and tubulointerstitial compartments. Microarray-derived differential gene expression of 88 genes representing critical enzymes of metabolic pathways and 25 genes related to immune cell markers was compared between disease groups. Correlation analyses measured relationships between metabolic pathways, kidney function and cytokine production.Results Reduced steady state levels of mRNA species were enriched in pathways of oxidative phosphorylation and increased in the pentose phosphate pathway (PPP) with maximal perturbation in AAV and SLE followed by NS, and least in LD. Transcript regulation was isozymes specific with robust regulation in hexokinases, enolases and glucose transporters. Intercorrelation networks were observed between enzymes of the PPP (eg, transketolase) and macrophage markers (eg, CD68) (r=0.49, p<0.01). Increased PPP transcript levels were associated with reduced glomerular filtration rate in the glomerular (r=−0.49, p<0.01) and tubulointerstitial (r=−0.41, p<0.01) compartments. PPP expression and tumour necrosis factor activation were tightly co-expressed (r=0.70, p<0.01).Conclusion This study demonstrated concordant alterations of the renal transcriptome consistent with metabolic reprogramming across different forms of glomerulonephritis. Activation of the PPP was tightly linked with intrarenal macrophage marker expression, reduced kidney function and increased production of cytokines. Modulation of glucose metabolism may offer novel immune-modulatory therapeutic approaches in rare kidney diseases.