PT - JOURNAL ARTICLE AU - Audo, R AU - Schreiber, K AU - Combe, B AU - Morel, J AU - Daien, C TI - P089 Characterisation of chemokine receptors and migration of regulatory B cells in patients with rheumatoid arthritis and in healthy donors AID - 10.1136/annrheumdis-2018-EWRR2018.105 DP - 2018 Mar 01 TA - Annals of the Rheumatic Diseases PG - A51--A51 VI - 77 IP - Suppl 1 4099 - http://ard.bmj.com/content/77/Suppl_1/A51.1.short 4100 - http://ard.bmj.com/content/77/Suppl_1/A51.1.full SO - Ann Rheum Dis2018 Mar 01; 77 AB - Introduction B cells are pathogenic players in the development of rheumatoid arthritis (RA). More recently, it has been shown that B cells can also have regulatory functions, mainly through the secretion of interleukin-10 (IL-10). We found a decrease of IL10 +B (B10) cells in patients with early Rheumatoid Arthritis (RA) and B10 were inversely correlated with disease activity (DAS28), inflammation and rheumatoid factor, suggesting a key role at the initial phase of RA. Chemokines and chemokine receptors (CR) orchestrate migration of immune cells in physiological and pathological conditions. We hypothesise that CR could be involved in B10 functions and characterisation.Objectives We aim to assess expression of CR on B10 and to understand their migration into the joint.Methods Peripheral blood mononuclear cells (PBMCs) from 10 RA patients and 10 controls (HC) were activated 24 hours with CpG to generate B10 cells. We compared expression of several CR between B10 cells and IL-10neg B cells by FACS analysis. For functional test, B10 cells and IL-10neg B were sorted by using secretion assay (Miltenyi) and the ability to migrate in response to CCL21, CCL22, CXCL11, CXCL12 or CXCL13 was evaluated by using migration assay in 5 µM Transwell chambers. The presence of B10 cells in RA synovial tissue was evaluated by immunohistochemistry.Results We found that B10 cells from HC differentially expressed several CR than IL-10neg B cells, There were a strong decrease of CXCR5 and CXCR7 expression, and a strong increase of CCR4 expression in B10 cells (p<0.001) compared to IL-10neg B cells. Functional impact of differential expression was tested by migration assay. Among chemokines tested,CXCL13, CXCR5-ligand, attracted more B10 cells than IL-10neg B cells (n=7). More importantly, our results suggest that CR receptor profile on B10 cells is different between HC and RA (n=10). Preliminary results showed that only a few B10 can be found in RA synovial tissue among B cells. One can hypothesised that the different expression profile of CR on B10 cells of RA patients might explain a defect in joint migration, and thus promoting uncontrolled inflammation.Conclusions B10 cells have a specific CR profil compared to IL-10neg B cells. The differential expression of CR on RA B10 compared to HC might explain a defect in joint migration. Understanding of B10 migration is an important issue and could be used in the future to drive regulatory B cells into the joints as a new therapeutic approach for RA.Disclosure of interest None declared