TY - JOUR T1 - Genome-wide DNA methylation analysis in multiple tissues in primary Sjögren's syndrome reveals regulatory effects at interferon-induced genes JF - Annals of the Rheumatic Diseases JO - Ann Rheum Dis SP - 2029 LP - 2036 DO - 10.1136/annrheumdis-2015-208659 VL - 75 IS - 11 AU - Juliana Imgenberg-Kreuz AU - Johanna K Sandling AU - Jonas Carlsson Almlöf AU - Jessica Nordlund AU - Linnea Signér AU - Katrine Braekke Norheim AU - Roald Omdal AU - Lars Rönnblom AU - Maija-Leena Eloranta AU - Ann-Christine Syvänen AU - Gunnel Nordmark Y1 - 2016/11/01 UR - http://ard.bmj.com/content/75/11/2029.abstract N2 - Objectives Increasing evidence suggests an epigenetic contribution to the pathogenesis of autoimmune diseases, including primary Sjögren's Syndrome (pSS). The aim of this study was to investigate the role of DNA methylation in pSS by analysing multiple tissues from patients and controls.Methods Genome-wide DNA methylation profiles were generated using HumanMethylation450K BeadChips for whole blood, CD19+ B cells and minor salivary gland biopsies. Gene expression was analysed in CD19+ B cells by RNA-sequencing. Analysis of genetic regulatory effects on DNA methylation at known pSS risk loci was performed.Results We identified prominent hypomethylation of interferon (IFN)-regulated genes in whole blood and CD19+ B cells, including at the genes MX1, IFI44L and PARP9, replicating previous reports in pSS, as well as identifying a large number of novel associations. Enrichment for genomic overlap with histone marks for enhancer and promoter regions was observed. We showed for the first time that hypomethylation of IFN-regulated genes in pSS B cells was associated with their increased expression. In minor salivary gland biopsies we observed hypomethylation of the IFN-induced gene OAS2. Pathway and disease analysis resulted in enrichment of antigen presentation, IFN signalling and lymphoproliferative disorders. Evidence for genetic control of methylation levels at known pSS risk loci was observed.Conclusions Our study highlights the role of epigenetic regulation of IFN-induced genes in pSS where replication is needed for novel findings. The association with altered gene expression suggests a functional mechanism for differentially methylated CpG sites in pSS aetiology. ER -