TY - JOUR T1 - Deficient production of IL-1 receptor antagonist and IL-6 coupled to oxidative stress in cryopyrin-associated periodic syndrome monocytes JF - Annals of the Rheumatic Diseases JO - Ann Rheum Dis SP - 1577 LP - 1581 DO - 10.1136/annrheumdis-2012-201340 VL - 71 IS - 9 AU - Sonia Carta AU - Sara Tassi AU - Laura Delfino AU - Alessia Omenetti AU - Salvatore Raffa AU - Maria Rosaria Torrisi AU - Alberto Martini AU - Marco Gattorno AU - Anna Rubartelli Y1 - 2012/09/01 UR - http://ard.bmj.com/content/71/9/1577.abstract N2 - Objective To determine whether dysregulated production of cytokines downstream of interleukin (IL)-1 participates in the pathophysiology of cryopyrin-associated periodic syndromes (CAPS). Methods Primary monocytes from patients with CAPS, unstimulated or after stimulation with lipopolysaccharide (LPS) and other Toll-like receptor (TLR) agonists, were examined for signs of stress and production of IL-1β, IL-1 receptor antagonist (IL-1Ra) and IL-6 in comparison with monocytes from patients with autoimmune diseases and from healthy donors. Results Unstimulated CAPS monocytes showed mild signs of stress including elevated levels of reactive oxygen species and fragmented mitochondria. Stress signs were worsened by TLR stimulation and eventually led to protein synthesis inhibition with strong impairment of production of cytokines downstream of IL-1, such as IL-1Ra and IL-6. These defects were not detected in monocytes from autoimmune patients and healthy donors. Conclusions The stress state of LPS-stimulated CAPS monocytes and the consequent inhibition of translation are likely to be responsible for the impaired production of IL-1Ra and IL-6. The deficient secretion of these cytokines coupled with increased IL-1β release explains the severity of the IL-1-related clinical manifestations and the predominant implication of innate immunity in CAPS. ER -