TY - JOUR T1 - Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis JF - Annals of the Rheumatic Diseases JO - Ann Rheum Dis SP - 924 LP - 927 DO - 10.1136/ard.2009.112813 VL - 69 IS - 5 AU - Albert Holvast AU - Aalzen de Haan AU - Sander van Assen AU - Coen A Stegeman AU - Minke G Huitema AU - Anke Huckriede AU - Cornelis A Benne AU - Johanna Westra AU - Abraham Palache AU - Jan Wilschut AU - Cees G M Kallenberg AU - Marc Bijl Y1 - 2010/05/01 UR - http://ard.bmj.com/content/69/5/924.abstract N2 - Background Both antibody and cell-mediated immune responses are involved in the defence against influenza. In Wegener's granulomatosis (WG), antibody responses to influenza vaccination appear to be similar to those in healthy controls, but cell-mediated responses have not been studied. Objective To determine whether cell-mediated responses to influenza vaccination in WG vary from those in controls. Methods Twenty-five patients with WG and healthy controls received subunit influenza vaccine. Peripheral blood mononuclear cells were obtained before and 1 month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon γ (IFNγ) ELISpot and intracellular cytokine staining for IFNγ, tumour necrosis factor and interleukin 2. Results Before vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. After vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4 T cells increased in patients and controls for A/H1N1 and A/H3N2. Conclusions Cell-mediated responses to influenza vaccination in patients with WG are comparable to those in healthy controls. Dutch Trials Register number NTR1130. ER -