PT - JOURNAL ARTICLE AU - Jiro Fujita AU - Tamotsu Takeuchi AU - Naomi Dobashi AU - Yuji Ohtsuki AU - Michiaki Tokuda AU - Jiro Takahara TI - Detection of anti-ADAM 10 antibody in serum of a patient with pulmonary fibrosis associated with dermatomyositis AID - 10.1136/ard.58.12.770 DP - 1999 Dec 01 TA - Annals of the Rheumatic Diseases PG - 770--772 VI - 58 IP - 12 4099 - http://ard.bmj.com/content/58/12/770.short 4100 - http://ard.bmj.com/content/58/12/770.full SO - Ann Rheum Dis1999 Dec 01; 58 AB - OBJECTIVES It has been suggested that the humoral immune system plays a part in the pathogenesis of pulmonary fibrosis. Although circulating autoantibodies to lung protein(s) have been suggested, few lung proteins have been characterised. The purpose of this study is to determine the antigen recognised by serum of a patient with pulmonary fibrosis associated with dermatomyositis.METHODS To accomplish this, anti-small airway epithelial cell (SAEC) antibody in a patient's serum was evaluated using a western immunoblot.RESULTS An autoantibody against SAEC was found, and the antigen had a molecular weight of 62 kDa. Using the patient's serum, clones from the normal lung cDNA library were screened and demonstrated that anti-SAEC antibody in the patient's serum was against ADAM (A disintegrin and metalloprotease) 10.CONCLUSION This is the first report that demonstrates the existence of anti-ADAM 10 antibody in a patient with pulmonary fibrosis associated with dermatomyositis.