TY - JOUR T1 - Interleukin 4 increases human synovial cell expression of VCAM-1 and T cell binding. JF - Annals of the Rheumatic Diseases JO - Ann Rheum Dis SP - 601 LP - 607 DO - 10.1136/ard.53.9.601 VL - 53 IS - 9 AU - H Shimada AU - K Eguchi AU - Y Ueki AU - M Nakashima AU - I Yamashita AU - Y Kawabe AU - M Sakai AU - H Ida AU - T Aoyagi AU - S Nagataki Y1 - 1994/09/01 UR - http://ard.bmj.com/content/53/9/601.abstract N2 - OBJECTIVE--The effects were studied of interleukin 4 (IL-4) on T cell-synovial cell adhesion and on the expression of adhesion molecules on the surface of synovial fibroblast-like cells. METHODS--The adhesion of T cells toward the synovial cells were measured by 51chromium-labelled adhesion assay. The expression of adhesion molecules on synovial cells were analysed by flowcytometry. RESULTS--Stimulation of synovial cells with IL-4 increased T cell-synovial cells adhesion in a time- and dose-dependent manner. IL-4 considerably enhanced the expression of VCAM-1 on the surface of synovial cells, but not the expression of ICAM-1 and ELAM-1. The combination of IL-1 beta and IL-4 had no effect on the expression of ICAM-1 or VCAM-1 on the surface of synovial cells. The increased adhesion of T cells to IL-4 stimulated synovial cells was inhibited significantly by adding anti-VCAM-1 or anti-CD29 monoclonal antibody. Furthermore, anti-VLA-4 alpha or the combination of anti-VLA-4 alpha and anti-VCAM-1 antibodies blocked completely T-cell binding to IL-4 stimulated synovial cells. CONCLUSIONS--These results suggest that the increased adhesion of T cells to IL-4-stimulated synovial cells is mediated by VLA-4/VCAM-1 pathway. ER -