RT Journal Article
SR Electronic
T1 High molecular weight glycosaminoglycans in AA type amyloid fibril extracts from human liver.
JF Annals of the Rheumatic Diseases
JO Ann Rheum Dis
FD BMJ Publishing Group Ltd and European League Against Rheumatism
SP 562
OP 566
DO 10.1136/ard.50.8.562
VO 50
IS 8
A1 Magnus, J H
A1 Kolset, S O
A1 Husby, G
YR 1991
UL http://ard.bmj.com/content/50/8/562.abstract
AB Glycosaminoglycans have previously been identified in extracts of AA type hepatic amyloid fibril from a patient with amyloidosis associated with juvenile rheumatoid arthritis. The macromolecular properties of these polysaccharides are described here in more detail. By gel filtration and ion exchange chromatography glycosaminoglycans in the form of high molecular weight free chains were shown to coisolate with water extracted amyloid fibrils. About 60% of these were characterised as galactosamines (chondroitin sulphate/dermatan sulphate), whereas the remaining 40% consisted of N-sulphated glucosamines (heparin/heparan sulphate). The amyloid associated glycosaminoglycans were not part of intact proteoglycans in the fibril extracts.