PT  - JOURNAL ARTICLE
AU  - Magnus, J H
AU  - Kolset, S O
AU  - Husby, G
TI  - High molecular weight glycosaminoglycans in AA type amyloid fibril extracts from human liver.
AID  - 10.1136/ard.50.8.562
DP  - 1991 Aug 01
TA  - Annals of the Rheumatic Diseases
PG  - 562--566
VI  - 50
IP  - 8
4099  - http://ard.bmj.com/content/50/8/562.short
4100  - http://ard.bmj.com/content/50/8/562.full
SO  - Ann Rheum Dis1991 Aug 01; 50
AB  - Glycosaminoglycans have previously been identified in extracts of AA type hepatic amyloid fibril from a patient with amyloidosis associated with juvenile rheumatoid arthritis. The macromolecular properties of these polysaccharides are described here in more detail. By gel filtration and ion exchange chromatography glycosaminoglycans in the form of high molecular weight free chains were shown to coisolate with water extracted amyloid fibrils. About 60% of these were characterised as galactosamines (chondroitin sulphate/dermatan sulphate), whereas the remaining 40% consisted of N-sulphated glucosamines (heparin/heparan sulphate). The amyloid associated glycosaminoglycans were not part of intact proteoglycans in the fibril extracts.