RT Journal Article
SR Electronic
T1 Detection of IgG rheumatoid factor by concanavalin A treatment and complement fixation with IgG rheumatoid factor.
JF Annals of the Rheumatic Diseases
JO Ann Rheum Dis
FD BMJ Publishing Group Ltd and European League Against Rheumatism
SP 240
OP 245
DO 10.1136/ard.35.3.240
VO 35
IS 3
A1 K Tanimoto
A1 T Moritoh
A1 T Azuma
A1 Y Horiuchi
YR 1976
UL http://ard.bmj.com/content/35/3/240.abstract
AB Concanavalin A (Con A) froms precipitates with carbohydrate-rich protein such as IgM, IgD, IgE, and IgA. Since IgG contains little carbohydrate and does not react with Con A, the activity of IgG-rheumatoid factor (RF) can be measured in the supernate of the Con A-treated serum. When the latex fixation test (LFT) and the sensitized sheep cell agglutination test (SSCA) were perfromed in the supernate for the detection of IgG-RF, LFT was positive in 32-1% of sera, out of 137 sera originally positive for LFT, and SSCA was positive in 18-5% of sera, out of 119 sera originally positive for SSCA. IgG-RF exhibited lower complement fixing ability than IgM-RF and correlated with agglutination titres of IgG-RF, while the CH50 of the original serum did not correlate with haemolytic activities of either IgM-RF or IgG-RF.