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Altered CD8+ T cell subpopulation in the bone marrow microenvironment of cynomolgus monkeys with spontaneous ankylosing spondylitis
  1. Tangming Guan1,
  2. Zhenhua Bian2,
  3. Hongbin Gao1,
  4. Yi He3,
  5. Junru Yuan1,
  6. Hongping Wan4,
  7. Shuangjie Tang4,
  8. Yongfeng Li4,
  9. Jianming Qiu4,
  10. Panyue Luo2,
  11. Xiaolan Ye1,
  12. Shi Liang1,
  13. Siyu Chen1,
  14. Nvlue Cai1,
  15. Yezhi Guan1,
  16. Jianxin Liu1,
  17. Zhibin Zhao5,
  18. Huanhuan Jia1,
  19. Wei Yang6,
  20. Wende Li1
  1. 1Guangdong Laboratory Animals Monitoring Institute, Guangzhou, Guangdong, China
  2. 2South China University of Technology, Guangzhou, Guangdong, China
  3. 3Department of Rheumatology and Immunology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, China
  4. 4Guangdong Blooming-spring Biological Technology Development Co., Ltd, Guangzhou, Guangdong, China
  5. 5Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China
  6. 6The Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China
  1. Correspondence to Professor Wende Li, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, Guangdong, China; lwd{at}gdlami.com; Dr Wei Yang; yangwei029{at}126.com; Dr Huanhuan Jia; 245089270{at}qq.com

https://doi.org/10.1136/ard-2024-226018

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    Ankylosing spondylitis (AS) is an inflammatory immune-mediated disease.1 2 The aetiology of AS is still unknown, and the lack of substitute animal models is a bottleneck restricting investigation of the mechanism and therapy of AS. Our group has identified dozens of spontaneous AS cynomolgus monkeys, highly similar to AS patients in epidemiology, clinical signs, imaging, blood routine, blood biochemistry, pathology, etc., which provides an excellent primate model for the study of AS pathogenesis.3

    Recently, studies have confirmed that CD8+ T cells play an important role in the development of AS.4 5 However, the expression and distribution of CD8+ T cells in axial joint lesions of AS patients have not been reported due to the difficulty in obtaining samples of axial joints of AS patients. Therefore, we first examined the expression of CD8+ T cells in axial joint of AS monkeys. We observed a significant increase in the proportions of CD8+ T cells at the vertebral bone marrow and intervertebral disc lesion sites in AS monkeys (figure 1A). Additionally, we noticed an increase in the proportion of CD4+ T and CD11b+ cells in AS monkeys (online supplemental figure S1).

    Supplemental material

    [ard-2024-226018supp001.pdf]

    Figure 1

    Tissue-resident memory-like CD8+ T cells exist in the bone marrow and intervertebral disc lesions of ankylosing spondylitis (AS) monkeys. (A) Representative H&E staining images of vertebral bone marrow and …

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    Footnotes

    • Handling editor Josef S Smolen

    • TG, ZB, HG and YH contributed equally.

    • Contributors TG, WY, HJ, HW, ZZ and WL designed the study. TG, WY, HG, YH, JY, ST, YL, JQ, ZB, PL, XY, SL, SC, NC, YG and JL processed samples and performed experiments. TG, WY, HG, ST, ZB and PL performed data analyses. TG, WY and ZZ wrote the main manuscript. All authors reviewed the manuscript. All authors revised and approved the manuscript for submission. TG, ZB, HG, and YH contributed equally to this work. WL as guarantor.

    • Funding This work is supported by National Key Research and Development programme of China (2021YFF0702600), Guangdong Provincial Platform Base and Science and Technology Infrastructure Construction Project (2021B1212060002), the Natural Science Foundation of Guangdong Province (2023A1515010470), Department of Science and Technology of Guangdong Province (2023A1515111103), the Key Scientific and Technological Programme of Guangzhou City (2023B03J1257) and the China Postdoctoral Science Foundation (2024M750588).

    • Competing interests None declared.

    • Patient and public involvement Patients and/or the public were not involved in the design, or conduct, or reporting, or dissemination plans of this research.

    • Provenance and peer review Not commissioned; externally peer reviewed.

    • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.