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Pause in immunosuppressive treatment results in improved immune response to SARS-CoV-2 vaccine in autoimmune patient: a case report
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  1. Basil Golding,
  2. Youri Lee,
  3. Hana Golding,
  4. Surender Khurana
  1. Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Silver Spring, Maryland, USA
  1. Correspondence to Dr Surender Khurana, FDA, Silver Spring, MD 20993, USA; Surender.Khurana{at}fda.hhs.gov

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Patients on immunosuppressive drugs have been excluded from studies of SARS-CoV-2 mRNA vaccines’ (mRNA-BNT162b2 and mRNA-1273) clinical trials that resulted in their Emergency Use Authorization in the USA and Europe. Since the initiation of the global vaccination campaign, it became apparent that patients on immunosuppressive drugs may not generate optimal immune response following vaccination.1 Prednisone and mycophenalate are potent inhibitors of immune responses. Prednisone acts at many levels of immunity including the innate and adaptive responses, whereas mycophenalate mainly targets T and B cells.2 These and other immunosuppressive drugs used to treat patients with autoimmune disorders and organ transplant patients were shown to significantly curtail antibody responses following SARS-CoV-2 mRNA vaccines.3–5

We report on a 75-year-old male patient with an underlying autoimmune disorder, myasthenia gravis, which was controlled after receiving high doses of prednisone and mycophenalate for 9 months. At his first SARS-CoV-2 vaccination, he was receiving 7.5 mg prednisone on alternate days and 3 g mycophenalate daily (figure 1A). He received two doses of Moderna mRNA-1273 vaccine with a 4-week interval between doses. Neutralisation titres were measured using a pseudovirus neutralisation assay (PsVNA) as described previously.6 No SARS-CoV-2 neutralising antibodies were detected in plasma at 4 weeks after the second mRNA-1273 vaccination. In the same assay, age-matched (65–77 years old) immunocompetent individuals (healthcare workers who were not on any immunosuppressive drugs and vaccinated with two doses of mRNA-1273 SARS-CoV-2 vaccine in Maryland) generated neutralising antibody titres (PsVNA50: 50% reduction in neutralisation titres) ranging between 1:451 and 1:3293 against the WA-1 strain following mRNA-1273 vaccination.

Figure 1

Effect of drug treatment and SARS-CoV-2 vaccination-induced immune response in a patient with myasthenia gravis. (A) Timeline of drug treatment profile and SARS-CoV-2 vaccination of the patient with myasthenia gravis. (B) SARS-CoV-2 neutralising antibody titres as determined by pseudovirus neutralisation assay (PsVNA) in 293-ACE2-TMPRSS2 cells with SARS-CoV-2 WA-1 strain, UK variant (B.1.1.7), Japan variant (P.1), Indian variant (B.1.617.1) or South African variant (B.1.351) as described previously.6 PsVNA50 (50% neutralisation titre) titre values are shown.

The patient was able to receive a second series of vaccinations with the Pfizer mRNA-BNT162b2 at 42 days and 63 days (first and second dose, respectively) following the last vaccination with mRNA-1273. Three weeks prior to the first dose of mRNA-BNT162b2, the mycophenalate dose was reduced from 3 g to 2 g daily. The day prior to the second dose with mRNA-BNT162b2 and for 3 subsequent days, the patient did not take any prednisone or mycophenalate. Thereafter (3 days post-second vaccination), the maintenance dose of 7.5 mg prednisone on alternate days and 2 g mycophenalate daily was resumed (figure 1A). No change in his clinical status related to the myasthenia gravis was observed during the study. Two weeks after the second mRNA-BNT162b2 vaccination, the patient’s plasma was used to measure virus neutralisation against SARS-CoV-2 vaccine-matched WA-1 strain and multiple variants of concern. Robust virus neutralising antibody titres were measured with the highest titre against the vaccine-matched WA-1 strain and lowest against the B.1.351 SARS-CoV-2 variant strain (figure 1B).

This case study exemplifies a strategy that could lead to better immune responses following SARS-CoV-2 vaccination in patients on prolonged immunosuppressive drugs. It is possible that the additional vaccination series received by the individual in this report may have been sufficient without reducing the immunosuppressive medications. Indeed, this approach is being evaluated in ongoing trials. In this regard, Werbel et al7 reported that 24 of 30 (80%) of organ transplant recipients on multiple immunosuppressive regiments showed no receptor-binding domain (RBD) antibody binding response after the first vaccination series with mRNA vaccines. Among those, 67% were still seronegative for SARS-CoV-2 RBD antibodies following a third vaccination with no treatment modification.7

Therefore, if the virus neutralisation titres after SARS-CoV-2 vaccination are undetectable or low, it may be reasonable to consider temporary supervised reduction in the dose of immunosuppressive drugs prior and during the time of a second vaccination, to allow recovery of B and T cell function and a robust immune response to vaccination. This may be associated with risk that autoimmune diseases may relapse, or transplant rejection may occur. Careful studies need to be performed to determine whether the risk:benefit profile favours a temporary decrease in immunosuppressive drugs to allow for a successful SARS-CoV-2 vaccination-induced immune response against COVID-19.

Ethics statements

Ethics approval

The study protocol was approved by the US Food and Drug Administration’s (FDA) Research Involving Human Subjects Committee (FDA-RIHSC-2020-04-02 (252)). This study complied with all relevant ethical regulations for work with human participants, and informed consent was obtained. Samples were collected from individual who provided informed consent to participate in the study. The participant consented and is a coauthor and helped write the manuscript. All assays performed fell within the permissible usages in the original informed consent.

Acknowledgments

We thank Keith Peden and Marina Zaitseva for review of the manuscript. We thank Carol Weiss for providing plasmid clones expressing SARS-CoV-2 spike variants.

References

Footnotes

  • Handling editor Josef S Smolen

  • Contributors SK and HG designed the research. BG and HG provided clinical specimens and unblinded clinical data. YL and SK performed the research. SK, HG and BG contributed to writing.

  • Funding Supported by FDA’s Medical Countermeasures Initiative (MCMi) grant #OCET 2021-1565 to SK.

  • Disclaimer The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products or organisations imply endorsement by the US government.

  • Competing interests None declared.

  • Patient and public involvement Patients and/or the public were involved in the design, or conduct, or reporting, or dissemination plans of this research. Refer to the Methods section for further details.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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