Article Text

Antibody response to a single dose of SARS-CoV-2 mRNA vaccine in patients with rheumatic and musculoskeletal diseases
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  1. Brian J Boyarsky1,
  2. Jake A Ruddy1,
  3. Caoilfhionn M Connolly2,
  4. Michael T Ou1,
  5. William A Werbel3,
  6. Jacqueline M Garonzik-Wang1,
  7. Dorry L Segev1,4,
  8. Julie J Paik2
  1. 1Surgery, Johns Hopkins University, Baltimore, Maryland, USA
  2. 2Rheumatology, Johns Hopkins Unveristy, Baltimore, Maryland, USA
  3. 3Infectious Diseases, Johns Hopkins University, Baltimore, Maryland, USA
  4. 4Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland, USA
  1. Correspondence to Dr Dorry L Segev, Epidemiology Research Group in Organ Transplantation, Johns Hopkins, Baltimore MD 21205, Maryland, USA; dorry{at}jhmi.edu

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The immune response to SARS-CoV-2 messenger RNA (mRNA) vaccines in patients with rheumatic and musculoskeletal diseases (RMD) is undefined because these individuals were largely excluded from phase I–III studies. To better understand the immune response to vaccination in this patient population, we studied the antibody response in patients with RMD who completed the first dose of SARS-CoV-2 mRNA vaccination.

Participants with RMD across the USA were recruited to participate in this prospective cohort via social media. Those with prior SARS-CoV-2 were excluded. We collected demographics, RMD diagnoses and immunomodulatory regimens and tested for SARS-CoV-2 antibodies at baseline and prior to the second vaccine dose. Antibody testing was conducted on the semiquantitative Roche Elecsys anti-SARS-CoV-2 S enzyme immunoassay (EIA) which tests for antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein.1 We evaluated the association between demographic/clinical characteristics and positive antibody response using Fisher’s exact test and Wilcoxon rank-sum test.

We studied 123 participants who received their first SARS-CoV-2 vaccination dose between 8 January 2021 and 12 February 2021; 52% underwent BNT162b2, and 48% underwent mRNA-1273 (table 1). The most common reported RMD diagnoses were inflammatory arthritis (28%), systemic lupus erythematosus (SLE) (20%), Sjogren’s syndrome (13%) and overlap connective tissue diseases(29%). Whereas 28% reported not taking immunomodulatory agents, the remainder reported regimens including non-biologic disease-modifying antirheumatic drugs (DMARDs) (19%), biologic DMARDs (14%) and combination therapy (37%).

Table 1

Demographic and clinical characteristics of study participants, stratified by immune response to the first dose of SARS-CoV-2 mRNA vaccine

At a median (IQR) of 22 (18-26) days after the first vaccine dose, 74% (binomial exact 95% CI, 65% to 81%) had a detectable anti-RBD antibody response (online supplemental table 1). Younger participants appeared more likely to develop an antibody response (p=0.06). No differences were detected between disease groups or overall immunomodulatory therapy categories. However, those on regimens including mycophenolate or rituximab were less likely to develop an antibody response (p=0.001 and p=0.04, respectively) (table 2). Nearly all patients (94%) on anti-tumour necrosis factor (TNF) inhibitor therapy had detectable antibodies.

Table 2

Participant immunomodulatory therapy,* stratified by humoral immune response to the first dose of SARS-CoV-2 mRNA vaccine

In this study of the immune response to the first dose of the SARS-CoV-2 mRNA vaccine in patients with RMD, the majority of participants developed detectable anti-SARS-CoV-2 RBD antibodies; however, patients on regimens including mycophenolate or rituximab were less likely to develop an antibody response. Overall, there were no major differences by diagnosis or being on immunomodulatory therapy (versus not being on therapy), though consistent with prior studies younger patients were more likely to develop antibody responses. Nearly all patients on anti-TNF therapy developed detectable antibody. These associations warrant further investigation.

Rituximab and methotrexate have been shown to reduce humoral responses to influenza and pneumococcal vaccines.2 3 We found that patients on rituximab were less likely to develop antibody response, yet methotrexate did not negatively impact antibody development. In addition, we found that patients on mycophenolate were less likely to develop antibody response to mRNA vaccination, consistent with observed experience of SARS-CoV-2 mRNA vaccination in the solid organ transplant population4 and reduced response to human papillomavirus vaccination in patients with SLE.5

Limitations of this study include a small, non-randomised sample; limited information on immunomodulatory dosage and timing; lack of serial measurements; and use of an EIA designed to detect antibody response after natural infection. Furthermore, these are data on the first-dose response to a two-dose series.

Nearly half of the patients with RMD have expressed hesitancy or unwillingness to receive a SARS-CoV-2 mRNA vaccine due to a paucity of data6; however, this report can provide reassurance to patients and their providers. We did, however, observe that certain lymphocyte-modulating therapies were associated with poorer humoral vaccine response; potential exploratory strategies to increase immunogenicity in this subgroup may involve adjustment in immunomodulatory therapy, dosage or timing around vaccination.

References

Supplementary materials

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Footnotes

  • DLS and JJP are joint senior authors.

  • BJB and JAR are joint first authors.

  • Handling editor Josef S Smolen

  • Twitter @BrianBoyarsky, @CaoilfhionnMD, @jgaronzikwang, @Dorry_Segev

  • Contributors All authors contributed equally to the manuscript.

  • Funding This research was made possible with generous support of the Ben-Dov family. This work was supported by grant numbers F32DK124941 (Boyarsky) and K23DK115908 (Garonzik‐Wang) from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), by K24AI144954 (Segev) from National Institute of Allergy and Infectious Diseases (NIAID), by K23AR073927 (Paik) from NIAMS and by grant gSAN-201C0WW from the Transplantation and Immunology Research Network of the American Society of Transplantation (Werbel). The analyses described here are the responsibility of the authors alone and do not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products or organisations imply endorsement by the US Government.

  • Competing interests DLS has the following financial disclosures: consulting and speaking honoraria from Sanofi, Novartis, CSL Behring, Jazz Pharmaceuticals, Veloxis, Mallincrodt and Thermo Fisher Scientific.

  • Patient consent for publication Not required.

  • Ethics approval This study was approved by the Johns Hopkins School of Medicine Institutional Review Board (IRB00248540).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.

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