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Semiquantitative analysis of line blot assay for myositis-specific and myositis-associated antibodies: a better performance?
  1. Ilaria Cavazzana1,
  2. Micaela Fredi1,2,
  3. Franco Franceschini1,2
  1. 1Rheumatology and Clinical Immunology Unit, ASST Spedali Civili of Brescia, Brescia, Italy
  2. 2Department of Clinical and Experimental Science, University of Brescia, Brescia, Italy
  1. Correspondence to Dr Ilaria Cavazzana, Rheumatology and Clinical Immunology, ASST Spedali Civili of Brescia, Brescia 25100, Italy; ilariacava{at}virgilio.it

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We read with interest the letter of Espinosa-Ortega F et al1 comparing line immunoblot assay (LIA) and immunoprecipitation (IP) performance in the detection of myositis-specific antibody (MSA) and myositis-associated antibody (MAA). The authors found a global moderate agreement between tests, with a very good agreement for anti-signal recognition particle (SRP), anti-Ku and anti-small ubiquitin-like modifier activating enzyme (SAE1); a good agreement for anti-hystidil-tRNA synthetase (Jo-1) and a moderate agreement for anti-PM/Scl, anti-melanoma differentiation-associated gene 5 (MDA5) and anti-transcription intermediary factor 1 (TIF1 gamma). …

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