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Response to: ‘Can solid-phase assays replace immunofluorescence for ANA screening?’ by Bizzaro
  1. David S Pisetsky1,
  2. Diane M Spencer1,
  3. Peter E Lipsky2,
  4. Brad H Rovin3
  1. 1 Department of Medicine and Immunology, Duke University Medical Center and Medical Research Service, VA Medical Center, Durham, North Carolina, USA
  2. 2 RILITE Research Institute, Charlottesville, Virginia, USA
  3. 3 Division of Nephrology, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
  1. Correspondence to Dr David S Pisetsky, Department of Medicine and Immunology, Duke University Medical Center and Medical Research Service, VA Medical Center, Durham, NC 27705, USA; david.pisetsky{at}duke.edu

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We would like to thank Dr Bizzaro for his commentary1 on our article2 on the variability of testing for antinuclear antibodies (ANA) by indirect immunofluorescence (IIF). Along with other letters that have been published in response to our article,3–9 Dr Bizzaro’s letter highlights the concerns about the IIF, its status as the ‘gold standard’ and the availability of other technologies (eg, solid phase assays) that …

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