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Response to: ‘Variation in antinuclear antibody detection by automated indirect immunofluorescence analysis’ by van Hoovels et al
  1. David S Pisetsky1,
  2. Diane M Spencer1,
  3. Peter E Lipsky2,
  4. Brad H Rovin3
  1. 1Department of Medicine and Immunology, Duke University Medical Center and Medical Research Service, Veterans Administration Medical Center, Durham, North Carolina, USA
  2. 2RILITE Research Institute, Charlottesville, Virginia, USA
  3. 3Division of Nephrology, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA
  1. Correspondence to Dr David S Pisetsky, Department of Medicine and Immunology, Duke University Medical Center, Durham, NC 27705, USA; david.pisetsky{at}

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We thank van Hoovels et al1 for their comments on our article2 on the variability of antinuclear antibody (ANA) determinations; other letters also discussed ANA testing issues.3 4 Meroni et al noted the value of distinguishing staining patterns by immunofluorescence assays (IFAs).3 …

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