Objective: To analyze the influence of mitochondrial DNA (mtDNA) haplogroups on serum levels of molecular biomarkers in patients with osteoarthritis (OA).
Methods: We analyzed serum levels of molecular biomarkers of cartilage metabolism (collagen type-II markers: Coll2-1, Coll2-1NO2, C2C, CPII), synovial metabolism (hyaluronic acid (HA)) and cartilage and synovial turnover (YKL-40) in 73 OA cases and 77 healthy controls using enzyme-linked immunosorbent assays (ELISAs). All subjects had been previously genotyped for the mtDNA haplogroups J, U and H. Non-parametric and multivariate analysis were performed to test the effects of the clinical variables, including gender, age, smoking status, diagnosis, mtDNA haplogroups and radiologic Kellgren/Lawrence (K/L) grade on the serum levels of the molecular markers.
Results: Non-parametric analysis found increased serum levels of HA in patients with OA, while the values for C2C and the C2C/CPII ratio were significantly higher in the healthy controls. A multiple regression analysis showed a relationship between the mtDNA haplogroups and serum levels of the typical collagen type II markers. Carriers of the mtDNA haplogroup H had higher levels while carriers of the mtDNA haplogroup J showed lower levels. Statistically significant interactions between mtDNA haplogroups and diagnosis and between mtDNA haplogroups and radiologic K/L grade in the serum levels of molecular markers were also found.
Conclusion A new role for mtDNA haplogroups emerges from this work. Our results suggest that the mtDNA haplogroups interact significantly with the serum levels of OA-related molecular markers, suggesting the possibility of their use as a complementary assay with these molecular markers.
Statistics from Altmetric.com
Review history and Supplementary material
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.