Statistics from Altmetric.com
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.
We have read with interest the paper of Tanaka et al 1 and support the need for a more personalised approach in dosing regimens of tumour necrosis factor (TNF) inhibitors.2 They adjusted infliximab dose based on TNF concentrations at baseline, under the assumptions that baseline TNF concentrations can be accurately measured, and that high disease activity (ie, more inflammation) is associated with higher TNF concentrations. However, several factors argue against these assumptions, which we would like to add to the study by Tanaka et al. These factors might explain the absence of an association between TNF concentrations and treatment response.
First, the quantification of TNF at baseline is in fact challenging: TNF is an unstable molecule with a short half-life.3 Baseline concentrations in circulation are very low, around the detection limit of most immunoassays, even during active disease. It is doubtful that baseline TNF concentrations can be measured with sufficient accuracy and precision to serve as basis for individualised treatment decisions including the adjustment of infliximab dose.
Second, they suggested that low infliximab trough levels in non-responding patients might be a consequence of excessive TNF production and resultant high TNF plasma concentrations. However, we have recently been able to quantify total circulating TNF during adalimumab and etanercept treatment in rheumatoid arthritis patients.4 5 On treatment, we observed an increase in circulating drug-bound TNF, reaching steady-state concentrations around 100–1000 pg/mL. This increase could be explained by a prolonged TNF half-life, due to its tight binding to the TNF inhibitor, which itself has a very long half-life. Nevertheless, steady-state TNF concentrations are still orders of magnitude lower than typical TNF inhibitor trough levels, also for infliximab. No association between TNF captured in circulation and concentrations of adalimumab and etanercept >1 µg/mL was observed.4 5 It is therefore unlikely that TNF induces target-mediated clearance of infliximab, explaining the lower infliximab concentrations in patients with high baseline TNF. Instead, we suppose that low infliximab concentrations are mainly the result of antidrug antibody (ADA) formation.6 Unfortunately, the association between serum drug concentration and ADA formation has not been addressed in the paper of Tanaka et al.
Finally, our recent data indicate that TNF concentrations in circulation are not at all reflective of (underlying) inflammation or disease activity, since steady-state TNF concentrations remained extremely stable for a follow-up of 2 years, irrespective of disease activity. Furthermore, to our surprise, we found a similar increase in circulating TNF in healthy volunteers who received a single dose of an adalimumab biosimilar.4 This suggests that the majority of TNF in circulation most likely does not originate from pathological processes.
The aim of the study by Tanaka et al to personalise treatment is of significant value. Our recent results give an explanation why circulating TNF might not be an appropriate biomarker for treatment response, like Tanaka et al showed. Instead, the use of therapeutic drug monitoring to optimise the dose in clinical practice is of growing interest and could significantly contribute to personalised treatment.2
Patient consent for publication
LCB and MJlA contributed equally.
Contributors LCB, MJlA, GJW and TR have all made substantial contributions to the conception, drafting and revising of the work. All authors approved the final version.
Funding This study was funded by ZonMw, 2Treat (Grant 436001001).
Competing interests GJW has received a research grant from Pfizer (paid to the institution) and honoraria for lectures from UCB, Pfizer, AbbVie, Biogen and BMS. TR has received honoraria for lectures from Pfizer, AbbVie and Regeneron, and a research grant from Genmab.
Provenance and peer review Not commissioned; internally peer reviewed.