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Utility of the HEp-2000 antinuclear antibody substrate
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  1. Adrian Y S Lee1,
  2. Dimitra Beroukas2,
  3. Peter J Roberts-Thomson1
  1. 1 Clinical Immunology and Allergy, Flinders Medical Centre, Bedford Park, South Australia, Australia
  2. 2 Immunology, SA Pathology, Bedford Park, South Australia, Australia
  1. Correspondence to Dr Adrian Y S Lee, Clinical Immunology and Allergy, Flinders Medical Centre, Bedford Park, South Australia, Australia; adrian.lee2{at}sa.gov.au

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We write with regards to the recently published consensus paper on antinuclear antibody (ANA) staining patterns by Damoiseaux et al which was most informative.1 We were surprised, however, that there was no mention or discussion of the HEp-2000 substrate (Immunoconcepts, Sacremento, California, USA) despite the acknowledgement that anti-SS-A/Ro60 is frequently missed on indirect immunofluorescence assay (IIFA).1 The HEp-2000 substrate is a modified HEp-2 substrate that is transfected with Ro60 cDNA …

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