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Semiquantitative analysis of line blot assay for myositis-specific and myositis-associated antibodies: a better performance?
  1. Ilaria Cavazzana1,
  2. Micaela Fredi1,2,
  3. Franco Franceschini1,2
  1. 1 Rheumatology and Clinical Immunology Unit, ASST Spedali Civili of Brescia, Brescia, Italy
  2. 2 Department of Clinical and Experimental Science, University of Brescia, Brescia, Italy
  1. Correspondence to Dr Ilaria Cavazzana, Rheumatology and Clinical Immunology, ASST Spedali Civili of Brescia, Brescia 25100, Italy; ilariacava{at}

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We read with interest the letter of Espinosa-Ortega F et al 1 comparing line immunoblot assay (LIA) and immunoprecipitation (IP) performance in the detection of myositis-specific antibody (MSA) and myositis-associated antibody (MAA). The authors found a global moderate agreement between tests, with a very good agreement for anti-signal recognition particle (SRP), anti-Ku and anti-small ubiquitin-like modifier activating enzyme (SAE1); a good agreement for anti-hystidil-tRNA synthetase (Jo-1) and a moderate agreement for anti-PM/Scl, anti-melanoma differentiation-associated gene 5 (MDA5) and anti-transcription intermediary factor 1 (TIF1 gamma). …

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