Statistics from Altmetric.com
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.
- systemic lupus erythematosus
- antiphospholipid syndrome
- autoimmune diseases
- sjøgren's syndrome
With much interest, we read the comments1 of our colleagues Yavuz and Rönnblom regarding our manuscript on galectin-9 as an easy to measure biomarker to detect the interferon (IFN) signature in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS).2 We thank them for their interest in our manuscript and their shared interest in the importance of the IFN signature in SLE and the subsequent need for more easily applicable markers to detect IFN activity. In the paper, we reported independently replicated correlations of galectin-9 with the IFN signature in patients with SLE and APS. The aim of our manuscript was to identify more easy to measure markers to detect the IFN signature. Besides serving as a marker for the IFN signature, we found that galectin-9 was elevated in patients with SLE and APS and correlated with signs of disease activity including SLEDAI.
We agree with the authors that the use of galectin-9 as a marker for IFN activity needs further confirmation before clinical implementation, and studies in which longitudinal samples and patients with different ethnic backgrounds are tested are among the next steps. Other candidates include the direct measurement of IFNs by digital ELISA,3 although these may only capture one subtype of IFN at a time.2 As galectin-9 expression is induced by IFNs, galectin-9 is elevated in several (viral) infectious diseases and, similar to the IFN signature, may not be helpful in distinguishing (viral) infections from SLE disease activity, as discussed by Yavuz and Rönnblom.
Additionally, the authors mention a protective effect of galectin-9 administration in murine models of SLE. Indeed, recent studies in lupus-like animal models support a role for galectin-9 in the pathogenesis of SLE.4 5 Its effects in these animal models however are ‘perplexing’,6 as the effects of a LGALS9 knock-out in pristane-induced lupus has opposite effects compared with galectin-9 administration in BXSB/MpJ and NZB/W F1 mice.4–6 Importantly, until our recent study, no data were available on galectin-9 levels in patients with SLE. Therefore, further studies to investigate the pathophysiological role of galectin-9 and studies in human patients with SLE are certainly relevant.
Besides SLE and APS,7 8 the IFN signature is present in several systemic autoimmune diseases including primary Sjögren’s syndrome (pSS), systemic sclerosis and (juvenile) dermatomyositis (JDM).9–11 In this regard, elevated levels of galectin-9 have also been reported in patients with JDM, correlating with treatment-induced changes in disease activity in longitudinal samples.12 In addition, we found elevated levels of galectin-9 in patients with pSS as compared with patients with non-Sjögren sicca, correlating with disease activity as assessed by ESSDAI (EULAR Sjögren’s Syndrome Disease Activity Index) and serum IgG levels (figure 1). Notably, in a randomised placebo-controlled trial in patients with pSS,13 we observed that successful disease inhibition by leflunomide/hydroxychloroquine combination therapy was associated with downregulation of galectin-9 levels (compared with no change in the placebo group) and changes in galectin-9 levels paralleled changes in markers of IFN activity, B-cell hyperactivity and disease activity (ESSDAI/ESSPRI) (manuscript in preparation). Therefore, serum levels of galectin-9 are a promising marker to assess the IFN signature and correlate with changes in disease activity in patients with different autoimmune diseases.
Handling editor Josef S Smolen
Contributors All authors contributed to the final draft of the manuscript.
Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.
Competing interests None declared.
Patient consent for publication Obtained.
Ethics approval METC UMC Utrecht.
Provenance and peer review Commissioned; internally peer reviewed.