You are here

Article Text

Article menu
Download PDFPDF
Correspondence
Antinuclear autoantibodies: discordance among four different assays
  1. Yovana Pacheco1,
  2. Diana M Monsalve1,
  3. Yeny Acosta-Ampudia1,
  4. Cilia Rojas1,2,
  5. Juan-Manuel Anaya1,
  6. Carolina Ramírez-Santana1
  1. 1 Center for Autoimmune Diseases Research (CREA), School of Medicine and Health Sciences, Universidad Del Rosario, Bogotá, Colombia
  2. 2 Clinical Laboratory CAYRE, Institute of Health Provider, Bogotá, Colombia
  1. Correspondence to Professor Carolina Ramírez-Santana, Center for Autoimmune Diseases Research (CREA), School of Medicine and Health Sciences, Universidad Del Rosario, Bogotá 111221, Colombia; heily.ramirez{at}urosario.edu.co

https://doi.org/10.1136/annrheumdis-2018-214693

Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    Detection of antinuclear antibodies (ANAs) is of paramount importance for diagnosis and assessment of autoimmune diseases (ADs), but may vary depending on the method used. Therefore, we aimed to compare three different line immunoassays (LIAs) for ANAs testing in order to simultaneously evaluate their concordance with the results obtained by a well known and widely used ELISA. Sera from women with established systemic lupus erythematosus (SLE, n=62) and Sjogren’s syndrome (SS, n=28) were examined. Patients with SLE had an average age of 48.7 years (IQR 36.8–57.0) with a disease duration of 14.8 years (IQR 9.0–18.0). Patients with SS had an average age of 62.1 years (IQR 55.5–71.0) with a duration of disease of 13.1 years (IQR 8.3–17).

    Table 1 shows the results from the two-by-two comparison for the four techniques. Different levels of agreement were observed among the assays. In general, they were deficient. The analysis showed only one ‘strong’ level of agreement between ELISA and IMTEC-ANA-LIA Maxx for anti-Ro measurement. Out of 39 comparisons, 14 did …

    View Full Text

    Linked Articles