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Correspondence
Antinuclear autoantibodies: discordance among four different assays
  1. Yovana Pacheco1,
  2. Diana M Monsalve1,
  3. Yeny Acosta-Ampudia1,
  4. Cilia Rojas1,2,
  5. Juan-Manuel Anaya1,
  6. Carolina Ramírez-Santana1
  1. 1 Center for Autoimmune Diseases Research (CREA), School of Medicine and Health Sciences, Universidad Del Rosario, Bogotá, Colombia
  2. 2 Clinical Laboratory CAYRE, Institute of Health Provider, Bogotá, Colombia
  1. Correspondence to Professor Carolina Ramírez-Santana, Center for Autoimmune Diseases Research (CREA), School of Medicine and Health Sciences, Universidad Del Rosario, Bogotá 111221, Colombia; heily.ramirez{at}urosario.edu.co

http://dx.doi.org/10.1136/annrheumdis-2018-214693

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    Detection of antinuclear antibodies (ANAs) is of paramount importance for diagnosis and assessment of autoimmune diseases (ADs), but may vary depending on the method used. Therefore, we aimed to compare three different line immunoassays (LIAs) for ANAs testing in order to simultaneously evaluate their concordance with the results obtained by a well known and widely used ELISA. Sera from women with established systemic lupus erythematosus (SLE, n=62) and Sjogren’s syndrome (SS, n=28) were examined. Patients with SLE had an average age of 48.7 years (IQR 36.8–57.0) with a disease duration of 14.8 years (IQR 9.0–18.0). Patients with SS had an average age of 62.1 years (IQR 55.5–71.0) with a duration of disease of 13.1 years (IQR 8.3–17).

    Table 1 shows the results from the two-by-two comparison for the four techniques. Different levels of agreement were observed among the assays. In general, they were deficient. The analysis showed only one ‘strong’ level of agreement between ELISA and IMTEC-ANA-LIA Maxx for anti-Ro measurement. Out of 39 comparisons, 14 did …

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    Footnotes

    • Handling editor Josef S Smolen

    • Contributors YP, DMM, YAA, JMA and CRS contributed to the design, the analysis of the results and to the writing of the manuscript. YP, DMM, YAA, CR and CRS performed the experiments.

    • Funding This work was supported by the Universidad del Rosario, grant number IV-FBG001.

    • Competing interests None declared.

    • Patient consent Obtained.

    • Ethics approval Universidad del Rosario.

    • Provenance and peer review Not commissioned; internally peer reviewed.

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