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Nature of T cell epitopes in lupus antigens and HLA-DR determines autoantibody initiation and diversification
  1. Zhenhuan Zhao1,
  2. Jiling Ren1,
  3. Chao Dai1,
  4. Carol C Kannapell1,
  5. Hongyang Wang1,
  6. Felicia Gaskin2,
  7. Shu Man Fu1,3
  1. 1 Division of Rheumatology, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA
  2. 2 Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, Charlottesville, Virginia, USA
  3. 3 Department of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, Virginia, USA
  1. Correspondence to Dr Shu Man Fu, Division of Rheumatology, Department of Medicine, University of Virginia, Virginia, Charlottesville, USA; sf2e{at}virginia.edu

Abstract

Objectives The generation of systemic lupus erythematosus (SLE)-related autoantibodies have been shown to be T cell dependent and antigen driven with HLA-DR restriction. In this study, the initiating antigen(s) and the mechanism of autoantibody diversification were investigated.

Methods T cell epitopes (T-epitopes) of SmD1 (SmD) were mapped by T-T hybridomas generated from DR3+AE0 mice immunised with SmD and with SmD overlapping peptides. TCRs from the reactive hybridomas were sequenced. The core epitopes were determined. Bacterial mimics were identified by bioinformatics. Sera from DR3+AE0 mice immunised with SmD peptides and their mimics were analysed for their reactivity by ELISA and immunohistochemistry. Samples of blood donors were analysed for HLA-DR and autoantibody specificities.

Results Multiple HLA-DR3 restricted T-epitopes within SmD were identified. Many T-T hybridomas reacted with more than one epitope. Some of them were cross-reactive with other snRNP peptides and with proteins in the Ro60/La/Ro52 complex. The reactive hybridomas used unique TCRs. Multiple T-epitope mimics were identified in commensal and environmental bacteria. Certain bacterial mimics shared both T and B cell epitopes with the related SmD peptide. Bacterial mimics induced autoantibodies to lupus-related antigens and to different tissues. HLA-DR3+ blood donors made significantly more SLE-related autoantibodies.

Conclusions The unique antigenic structures of the lupus-related autoantigens provide the basis for being targeted and for T and B cell epitope spreading and autoantibody diversification with unique patterns. SLE-related autoantibodies are likely generated from responses to commensal and/or environmental microbes due to incomplete negative selection for autoreactive T cells. The production of SLE-related antibodies is inevitable in normal individuals. The findings in this investigation have significant implications in autoimmunity in general.

  • SLE
  • T cell epitopes
  • molecular mimicry
  • HLA-DR
  • autoantibody
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Footnotes

  • ZZ and JR contributed equally.

  • Handling editor Josef S Smolen

  • Contributors ZZ, JR, CD and CCK designed and carried out most of the experiments. HW did the histological experiments. FG did serological experiments and contributed to the writing of the manuscript. ZZ and CD wrote the unpublished software for the identification of potential DR-3-restricted bacterial SmD T cell mimics. SMF designed experiments, directed the project and wrote the manuscript with comments from all authors.

  • Funding This research was supported by grants from the National Institutes of Health: specifically, R01 AR047988 from the National Institute of Arthritis and Skin and Musculoskeletal Diseases and from the National Institute of Diabetes and Kidney Diseases R01 DK105833. It was also supported by the Alliance for Lupus Research TIL332615 and Lupus Research Alliance DIA481517.

  • Disclaimer The opinions in this manuscript do not reflect those of the granting agencies.

  • Competing interests None declared.

  • Patient consent Not required.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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