Objectives NFIL3 is a key immunological transcription factor, with knockout mice studies identifying functional roles in multiple immune cell types. Despite the importance of NFIL3, little is known about its function in humans.
Methods Here, we characterised a kindred of two monozygotic twin girls with juvenile idiopathic arthritis at the genetic and immunological level, using whole exome sequencing, single cell sequencing and flow cytometry. Parallel studies were performed in a mouse model.
Results The patients inherited a novel p.M170I in NFIL3 from each of the parents. The mutant form of NFIL3 demonstrated reduced stability in vitro. The potential contribution of this mutation to arthritis susceptibility was demonstrated through a preclinical model, where Nfil3-deficient mice upregulated IL-1β production, with more severe arthritis symptoms on disease induction. Single cell sequencing of patient blood quantified the transcriptional dysfunctions present across the peripheral immune system, converging on IL-1β as a pivotal cytokine.
Conclusions NFIL3 mutation can sensitise for arthritis development, in mice and humans, and rewires the innate immune system for IL-1β over-production.
- juvenile idiopathic arthritis
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Homozygous NFIL3 mutations identified in monozygotic twins with juvenile idiopathic arthritis.
Enhanced susceptibility to arthritis induction in Nfil3-knockout mice.
NFIL3 loss in patients and mice is associated with elevated production of IL-1β.
Knockdown of NFIL3 in healthy macrophages drives IL-1β production.
Juvenile idiopathic arthritis (JIA) is the most common of the childhood rheumatic diseases. JIA is characterised as juvenile-onset persistent arthritis with no defined cause. A high degree of clinical heterogeneity is observed within the JIA group of diseases, thought to reflect a diversity in genetic and environmental factors and mechanistic drivers. JIA shows similarities to adult autoimmune diseases, and, indeed, genome-wide association studies identify a strong overlap in the common variants linked to autoimmune susceptibility and JIA susceptibility.1 JIA also has similarities to autoinflammatory diseases, such as genetic associations to innate inflammatory pathways2 and response to IL-1β blockade.3 The recent success in identifying monogenic causes of autoinflammatory diseases4 suggests that monogenic causes may also underlie a subset of patients with JIA. Indeed, the association of systemic JIA with mutations in LACCI 5 supports the potential productivity of this approach in the non-systemic JIA diseases.
NFIL3 is an important transcription factor in the immune system. Analysis of Nfil3-deficient mice has identified a key role for Nfil3 in the development of natural killer (NK) cells,6 with similar functions in other innate lymphoid cells,7 and the CD8α+ dendritic cell subset.8 Within T cells, Nfil3 enhances the Th2 lineage9 while suppressing the Th17 lineage.10 The net effect of these changes is the spontaneous development of colitis, a process dependent on microflora and T cell activation.11 Less is known about the function of NFIL3 in humans. Expression of NFIL3 is reduced in patients with Crohn’s disease and ulcerative colitis,12 and in vitro gene silencing of NFIL3 in T cells and B cells promotes self-reactivity.13 These results suggest that NFIL3 plays a key immune homeostatic role in humans; however, genetically deficient patients are required to understand the in vivo function.
Here, we have characterised monozygotic twins with JIA and inflammatory complications, who harbour homozygous mutations in NFIL3. Parallel studies in mice confirm the arthritogenic potential of NFIL3-deficiency, with Nfil3 knockout mice showing enhanced susceptibility to arthritis induction. Mechanistic analysis identified elevated production of IL-1β and TNFα by myeloid cells in the peripheral blood of NFIL3 patients and the inflamed joints of Nfil3-deficient mice. Our results here demonstrate a link between NFIL3 mutation and restraint of inflammatory cytokine production in the myeloid lineage, contributing to a monogenic form of JIA.
The study was approved by the Ethics Committee of UZ Leuven, Belgium, and written informed consent was obtained from the parents of the patients and age-matched healthy individuals. The study was performed in accordance with the modified version of the Helsinki declaration. Whole exome sequencing was performed as previously described on P1 and P2.14 Only coding non-synonymous variants with genotype quality >60, gene damage index score of <12 40515 and mean allele frequency of <0.005 in NHLBI GO Exome Sequencing Project 6500, 1000 Genomes Project (October 2014) or in The Exome Aggregation Consortium database were considered.
Human macrophage differentiation
Peripheral blood mononuclear cells (PBMCs) were isolated from heparinised blood using lymphocyte separation medium (LSM, MP Biomedicals). PBMCs were plated in Poly-D-Lysine coated flask in complete RPMI (Thermo Fisher Scientific) and incubated for 2 hours to enrich the monocyte population by plastic adherence. Monocytes were differentiated into macrophages for 7–10 days in differentiation media containing complete RPMI medium supplemented with recombinant hM-CSF (50 ng/mL) and hIL-10 (25 ng/mL) (both BioLegend), replacing media every 2–3 days during the differentiation period. Macrophages were transfected with either scrambled siRNA (SantaCruz, sc-36869, 30 pmol) or three siRNA targeting Nfil3 mRNA (ThermoFisher assay IDs 144020, 115 656 and 115655, 30 pmol each) by nucleofection (Lonza, VPA-1008, Nucleofector program T-016). Twenty-four hours post-transfection cells were stimulated with 1 µg/mL lipopolysaccharide (LPS) for 24 hours.
PBMCs were isolated using LSM (MP Biomedicals) from patients and healthy individuals. For intracellular staining, cells were plated with complete RPMI containing phorbol myristate acetate (PMA 50 ng/mL; Sigma-Aldrich), ionomycin (500 ng/mL; Sigma-Aldrich) and Brefeldin A (8 ng/mL; Tocris Bioscience) for 4 hours. Cells were fixed and permeabilised with the eBioscience Foxp3 staining kit (eBioscience). Anti-human antibodies included anti-NFIL3 (REA732) (Miltenyi Biotec), anti-CD14 (TuK4) (eBioscience); anti-CD3 (Miltenyi Biotec); anti-CD16 (3G8), anti-CD56 (NCAM16.2), anti-CD123 (7G3), anti-CD27 (L128), anti-CD45RA (HI100), anti-CD8 (SK1), anti-CD4 (SK3), anti-CD1c (L161), anti-IFNγ (4S.B3), anti-T-BET (4B10), anti-IL-17a (N49-653), anti-GATA3 (L50-823) (all from BD Biosciences); anti-HLA-DR (L243), anti-CD19 (HIB19), anti-CD56 (NCAM16.2), anti-CD11c (3.9), anti-CCR7 (G043H7), anti-FOXP3 (206D), anti-RORγt (Q21-559), anti-TNFα (MAb11), anti-IL-4 (MP4-25D2) (all from BioLegend); purified Rabbit-anti-human NFIL3 (D5K8O) (Cell Signaling Technology) followed by Donkey-anti-Rabbit-IgG (Thermo Fisher Scientific). Data were collected on BD Symphony (BD Biosciences) and analysed using FlowJo V.10.5 (Tree Star Inc.).
Lysates from lymphoblastoid cells were run on the NuPAGE Precast Gel System (Life Technologies). Thirty to 50 µg of lysate were separated on 4%–12% bis-tris acrylamide gels and blotted on a PVDF membrane (GR Healthcare). Membranes were incubated with rabbit anti-NFIL3 (1:500, D5K80, Cell signaling) and mouse anti-Vinculin (1:2000, V9264, Sigma). Proteins were revealed using western Lightning Prime-ECL (GE Healthcare) and the imaging system G:Box XRQ (Syngene). Quantification was performed using the AIDA software (Raytest, V.5.0).
N-terminally FLAG-tagged human NFIL3-T2A-GFP (WT or carrying the M170I mutation) was expressed transiently from a plasmid in HEK293T. The expression was driven by chicken actin promoter with the CMV enhancer. For transfections, HEK293T cells were grown on poly-L lysine-treated (0.1%) cover slips to subconfluency. Plasmid transfection was done using Lipofectamine 3000 according to the manufacturers protocol (Thermo Fisher). Twenty-four hours after transfection, the cells were washed in PBS, fixed in 4% PFA and permeabilised in 0.1% Triton X-100 (in PBS). After blocking in PBS with 2% bovine serum albumine (BSA), 10% donkey serum and 0.1% Triton X-100 for 30 min, cells were stained with an anti-Flag polyclonal affinity antibody (F7425; Sigma Aldrich) for 2 hours, then washed and incubated for 1 hour with Alexa Fluor 555 donkey-anti-rabbit (A31572; Molecular Probes) antibody as well as DAPI (D1306; Molecular Probes). After washing the cells, they were covered using Fluoromount (Thermo Fisher). Images were collected on an LSM 510 Meta confocal microscope (Ziess) with a 60× immersion objective. Quantification of mean fluorescence intensity was measured using ImageJ software. Alternatively, 24 hours post-transfection, cells were stained with fixable viability dye (eBioscience), fixed and stained for human NFIL3 following the eBioscience protocol for flow cytometry analysis.
Arthritis induction in mice
C57Bl/6 and Nfil3 -/- mice16 were bred and housed under barrier conditions at a specific pathogen-free facility at the Walter and Eliza Hall Institute Animal Facility. Eight-to-ten week-old mice were used for all experiments. All procedures were approved by the Walter and Eliza Hall Institute Animal Ethics Committee. Serum transfer arthritis was induced by injection of arthritogenic serum from 12-week-old progeny of KRN and non-obese diabetic mice (K/BxN mice).17 Clinical score was assessed as a sum of the clinical score for each paw (0, no erythema and swelling; 1, mild erythema and swelling confined to the ankle, wrist or digits; 2, mild erythema and swelling extending from the ankle to the mid-foot; 3, moderate erythema and swelling extending from the ankle to the metatarsal joints; 4, severe erythema and swelling extending the entire limb and with joint ankylosis). The severity of joint inflammation was also assessed with in vivo imaging of bioluminescence using luminol, a substrate for myeloperoxidase activity (in myeloid cells), on days 4 and 7, as published previously.18 Arthritis of the ankle joint was evaluated histologically from two independent experiments. Front and hind limbs of mice were fixed in 10% neutral-buffered formalin, embedded in paraffin, sectioned at 7 µm and stained with Safranin-O, according to standard practices. Histological analysis was performed on serial joint sections. Histology scores are as follows: 0=normal, 1=moderate, 3=severe.
Flow cytometry was performed on cells isolated from the peritoneal lavage, joints and blood. For cytokine production measurement, cells were stimulated with LPS (0.1 µg/mL) in the presence of Brefeldin A and monensin for 3 hours, stained for surface markers, followed by intracellular staining of IL-1β and TNF.
Single cell sequencing
Peripheral blood was collected by venipuncture, and the PBMC fraction was isolated using LSM-Lymphocyte Separation Medium (MP Biomedicals). PBMC were then viably frozen and stored in liquid nitrogen prior to single cell sequencing. On thawing, the PBMC were counted using a Countess II Automated Cell Counter (Thermo Fisher), and 8700 cells for each sample were loaded individually onto the Chromium Controller (10x Genomics).
Analysis of single-cell RNA-seq data from patient and control PBMCs
Sequence data were preprocessed with Cell Ranger V.2.0 (10x Genomics). The resulting count matrices were analysed with R V.3.4 and the package Seurat V.2.2 (6), following the standard pipeline with default parameters, unless stated otherwise. Genes detected in less than five cells as well as cells with less than 500 genes detected were filtered out, leaving 15 216 genes across 4743 cells in the control and 14 367 genes across 2165 cells in the patient. Gene expression was normalised across genes by dividing by the total expression per cell, log-transformed and standardised across cells. The 1000 most variable genes were used to align the expression levels of both samples, through the components of a canonical correlation analysis (CCA). The tSNE plots were calculated on the first 20 components of the CCA, and clusters were identified by the community-detection algorithm implemented by Seurat.
Gene set enrichment analysis was carried out for each cluster (cell type) with GSEA v 3.0 (Broad Institute, Cambridge, Massachusetts, USA) (7). Gene sets with size larger than 1000 or smaller than 10 were excluded. Detection of variation in gene sets was controlled to have a false discovery rate lower than 0.25. Gene sets were prioritised according to the normalised enrichment score provided by GSEA.
KEGG pathways19 were analysed with Pathview,20 through the web server API. First, each cluster (cell type) was analysed separately, using all genes with detected fold-change, for the pathways corresponding to signal transduction, immune system and immune diseases. Then, the final pathway representation was obtained by merging the expression levels of the genes directly related to each cell type.
Real time PCR
RNA has been isolated from sorted CD14+ monocytes, differentiated macrophages or NIH3T3 cells using the ReliaPrep RNA Cell Miniprep System (Promega). cDNA synthesis was performed using the Superscript III RT System (Thermos Fisher). Expression of STX11, TGFB1, CSF2RB, CEBPG, CD224, NFIL3, TNF, IL1B, HPRT, RPL0, ACTB and plasmid-encoded ncRNA was measured by PrimeTime qPCR Probe Assays (IDT) and IL1B by SYBR green qPCR (Thermo Fisher). The expression of HPRT, RPL0 and ACTB was used to normalise mRNA expression.
NFIL3 mutations in monozygotic twins with juvenile idiopathic arthritis
Monozygotic twins were identified with JIA (figure 1A). Both sisters were diagnosed with oligoarticular JIA at the age of 4 years (P1) and 6 years (P2), respectively. Systemic inflammation at onset (sedimentation 49;<20 mm/hour, CRP 15.2; <5 mg/L, IgG 14.8; 4.78–11.29 g/L (P1); sedimentation 32; <20 mm/hour, CRP 2.9; IgG 17.50; 5.58–12.54 g/L (P2)) and antinuclear antibodies (table 1) were present in both. There was no occurrence of uveitis. Autoimmune thyroiditis developed at age 9 years (P1) and 11 years (P2). P1 was initially treated with intra-articular steroids and methotrexate, with adalimumab added after a relapse. At 11 years of age, laboratory tests revealed mildly increased liver enzymes with normal bilirubin levels, normal NSE, αFP and coagulation tests. Liver ultrasonography with duplex Doppler showed a large well-marginated lesion in the left liver lobe displacing the left subhepatic vein, with a characteristic spoke wheel vascularisation pattern compatible with focal nodular hyperplasia. MRI confirmed a T2 isointense multilobulate tumour with a central T2 hyperintense scar, occupying the left liver lobe. Methotrexate and adalimumab were stopped, with subsequent normalisation of liver enzymes. Over the course of the following 2 years, the hepatic lesion has remained stable but she recently suffered another relapse of knee arthritis. P2 remains in clinical remission after a course of non-steroidal anti-inflammatory drugs and an intra-articular steroid infiltration. The rare occurrence of JIA and autoimmune thyroiditis in monozygotic twins with a family history of psoriatic arthritis in the maternal grandmother, suggested a genetic driver of disease. The co-occurrence of focal nodular hyperplasia of the liver, not previously reported in patients with JIA, was also indicative of a systemic immune defect.
Genetic analysis of the patients through whole exome sequencing identified a homozygous mutation in NFIL3. Genetic variants were filtered for rare coding mutations. Based on the family history, recessive inheritance was deemed most likely. The patients were found to harbour one rare coding mutation in homozygosity, a G510A mutation in NFIL3, resulting in a methionine to isoleucine mutation at residue 170 (M170I). The mutation was confirmed by Sanger sequencing as homozygous in the affected patients and heterozygous in the parents (figure 1B). The mutation is in the Ser-rich region (figure 1C), in a highly conserved stretch of amino acids (figure 1D). Patient cell lines demonstrated a ~50% reduction NFIL3 expression at the protein level (figure 1E). In ex vivo primary cells, taken from an inflammatory environment, NFIL3 mRNA was increased; however, a ~50% reduction in the mRNA/protein ratio was observed (online supplementary figure S1). To formally test protein stability, we transfected cell lines with either the wildtype or M170I form of NFIL3 and observed 50% lower expression of the M170I allele (online supplementary figure S2). Together, these results indicate that M170I NFIL3 is unstable, without excluding additional functional loss from the amino acid change.
NFIL3 knockout mice have enhanced susceptibility to arthritis induction
In the absence of a second family with NFIL3 mutations, we turned to a mouse model. Nfil3 knockout mice have been previously characterised as possessing a diverse set of immunological alterations.6–11 Here, we challenged 8–10-week-old C57BL/6 and Nfil3 gene deleted mice with arthritogenic serum antibodies derived from the K/BxN mouse strain. This model bypasses early priming stages and compares sensitivity to downstream arthritis pathology processes. Compared with wildtype mice, Nfil3 knockout mice developed inflammatory arthritis earlier and had more severe joint inflammation, as assessed clinically (figure 2A), by in vivo imaging figure 2A-C and histologically (online supplementary figure S3). Investigation of the inflamed joints of wildtype and Nfil3 knockout mice identified an elevated myeloid infiltrate, dominated by neutrophils (figure 2D,E). Infiltrating neutrophils and monocytes/macrophages demonstrated enhanced production of IL-1β and TNF in the Nfil3 knockout joint (figure 2F,G). These changes in the joint were reflected in the serum, with elevated IL-1β and TNF in the arthritic Nfil3 knockout mice (figure 2H). Together, these results support NFIL3 as a genetic contributor to inflammatory arthritis in the patient pedigree and identify innate inflammatory cytokines as a potential mechanism.
NFIL3 mutations drive elevated IL-1β production in myeloid cells
In order to determine the immunological impact of NFIL3 loss of function on the peripheral immune system, we ran a single cell sequencing experiment on P1 and a healthy age-matched control. After data curation, data from 4743 cells from the healthy individual and 2165 cells from the patient were clustered using a tSNE approach (figure 3A). Clusters were manually annotated into leucocyte populations based on the expression of key lineage markers (online supplementary figure S4). Quantification of the clustered leucocyte populations revealed multiple immunological abnormalities in the patient (figure 3B,C). The adaptive immune system gave indications of defective activation, with increased naïve B cells and T cells, while memory B cells and activated T cells were normal and activated CD8 T cells were reduced. Changes were also observed in the innate immune system, with a shift from the CD14+ monocyte cluster to the CD16+ monocyte cluster, a relative defect in the CD56bright NK cluster and reduced frequencies of both CD1+ DC and pDC (figure 3C).
To validate the changes observed using single cell sequencing, we used a flow cytometric analysis on both P1 and P2 and four healthy controls (figure 4). As was observed using single cell sequencing, the innate immune system was disturbed in the NFIL3 patients, with an increased frequency of CD16+ monocytes (figure 4B) and a selective reduction in the CD56bright NK population (figure 4C). Analysis of T cell populations with flow cytometry picked up an increase in T cell activation not apparent at the transcript level. Th1, Th17, IFNγ-producing CD8 and TNF-producing CD8 T cells were all increased (figure 4G,I–K). These results validated and extended the single cell analysis, identifying an inflammatory milieu in NFIL3 patients.
Beyond changes in leucocyte population frequency, we used the single cell data to detect altered transcriptional pathways in NFIL3 patients. Global transcriptional analysis indicated differential effects of NFIL3 deficiency of each population (online supplementary figure S6). Naïve T cells and CD56dim NK cells were dominated by an upregulation of ribosomal components and protein production machinery. By contrast, activated T cells and B cells demonstrated increased expression of multiple transcription factors, including FOS, MYC, IRF1 and STAT3 (online supplementary figure S6), indicating stronger levels of activation. Many biological pathways were altered in the myeloid compartment, with the upregulation of components of the MAPK pathway the key feature (online supplementary figure S6), again indicative of excessive activation. When transcriptional changes were mapped onto the Rheumatoid Arthritis KEGG pathway, excessive production of IL-1β and TNF by innate leucocytes was identified as a key change (figure 5A), corresponding with the changes observed in mice (figure 2). Due to the known arthritogenic role of IL-1β, we tested whether a direct link could be established between NFIL3 expression in macrophages and IL-1β production. Using an siRNA approach, we knocked down NFIL3 expression in primary macrophages cultured from a healthy individual and found that ~50% reduction in NFIL3 primed macrophages for excessive IL-1β and TNF expression (figure 5B). This mechanistic analysis suggests that the effects of NFIL3-deficiency may be pleiotropic, with differential rewiring of multiple leucocyte populations culminating in dysregulated IL-1β and TNF production in an arthritogenic reaction.
In this study, the in vivo immunological role of NFIL3 has been characterised, with deficiency in NFIL3 sensitising to arthritis development in mice and in patients. Mechanistic analysis in both species converged on IL-1β overproduction by innate leucocytes as a potential disease mechanism. It is likely, however, that the effect of NFIL3 is more pleiotropic, with multiple complex interactions. For example, the adaptive immune system in these patients also demonstrated a Th1/Th17 skew, which may also contribute to disease. A proinflammatory phenotype of NFIL3 deficiency is consistent with both the murine model, which develops colitis,11 and correlative data in humans, where NFIL3 expression is reduced in patients with colitis.12 While the patients described here have not presented with colitis, it is increasingly recognised that the clinical presentation of autoinflammatory diseases is diverse, with the underlying biological defect manifesting as different clinical symptoms in different individuals. The identification of NFIL3 as an autoinflammatory gene opens up further investigation of monogenic patients, who may present with inflammatory phenotypes across the spectrum.
Independent of the role of NFIL3 mutations in disease, the identification of an NFIL3-deficient family allows the first analysis of the in vivo functions of NFIL3 in humans. In vitro gene silencing on NFIL3 in human T cells and B cells has been performed;13 however, in vivo experiments on NFIL3-deficiency have been restricted to mice. Comparison of the NFIL3-deficient patients assessed here with the Nfil3-deficient mice reveals both cross-species similarities and species-specific functions. The patients, as with the mice,6 have defects in NK cells, with a reduction in maturation to the CD56bright population. Likewise, in patients, NFIL3 deficiency results in a major loss of the cDC population, phenocopying mice.8 Here, we demonstrated a mechanistic link between NFIL3 expression and proinflammatory cytokine production, and an association between NFIL3 deficiency with arthritis in mice and patients.
Handling editor Josef S Smolen
Correction notice This article has been corrected since it published Online First. The corresponding author's details have been updated.
Contributors SS, EP, OB, TP, CS, CL, JD, SJ and KL performed experiments. SS, VL, CPR, IPW, GB and AL analysed data. EVN, SH-B and CW provided clinical information. SH-B, CW and AL designed and led the study.
Funding This work was supported by the ERC grant IMMUNO and the VIB Grand Challenges Program. SH-B, EP, VL, SJ and EVN are FWO fellows. This work was supported by the Reid Charitable Trusts, National Health and Medical Research Council of Australia Clinical Practitioner Fellowship (1023407), Senior Principal Research Fellowship (1135898), RD Wright Career Development Fellowship (1123000), Program Grants (1016647, 1054925) and Victorian Government Operational Infrastructure Support.
Competing interests None declared.
Patient consent for publication Not required.
Ethics approval UZ Leuven Ethical Committee.
Provenance and peer review Not commissioned; externally peer reviewed.
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