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P097 ANTI-TNF treatment improves vascular function via suppression of GALECTIN-3 expression during inflammatory arthritis
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  1. K Sime,
  2. E Hughes,
  3. EH Choy,
  4. AS Williams
  1. Cardiff University, Cardiff, UK

Abstract

Introduction The link between rheumatoid arthritis (RA) and cardiovascular disease (CVD) is well established but not yet fully understood. Anti-TNF treatment, e.g. with Enbrel, is one of most common therapies used in RA patients and has been shown to have beneficial effects on RA-associated cardiovascular pathologies in these patients.1 Initial studies in the collagen-induced arthritis (CIA) model revealed an increase in cardiac fibrosis marker galectin-3, both locally in perivascular adipose tissue (PVAT) and systemically in blood plasma during inflammatory arthritis and correlated with impaired vascular function.2

Objectives This study aims to investigate the impact of anti-TNF treatment on galectin-3 expression and vascular function during CIA.

Methods Male DBA/1 mice underwent CIA induction and received either Enbrel or phosphate buffered saline (PBS) by intravenous injection. Vascular function was determined by measuring the constriction response of the thoracic aorta at termination using a myograph. Galectin-3 was measured locally in thoracic PVAT by immunohistochemistry and quantitative polymerise chain reaction (qPCR), and systemically by ELISA.

Results Anti-TNF treatment during CIA resulted in a decrease in arthritis severity and progression compared to PBS treated mice, and partially restored vascular function. Galectin-3 expression was significantly reduced in thoracic PVAT, but not in blood plasma, in CIA mice treated with Enbrel.

Conclusions In conclusion, decreased galectin-3 expression in Enbrel treated mice is associated with an improvement in arthritis-associated disease activity including arthritis clinical score, joint swelling and vascular function.

References

  1. . Allanore Y, Avouac J. Cardiovascular risk in rheumatoid arthritis: Effects of anti-TNF drugs. Expert Opin Pharmacother2008May;9(7):1121–8. doi:10.1517/14656566.9.7.1121

  2. . Sime K, Choy EH, Williams AS. Alterations to adipose tissue morphology during inflammatory arthritis is indicative of vasculopathology in DBA/1 mice. Adipocyte2017April 3;6(2):87–101. doi:10.1080/21623945.2017.1295174 (Accessed: 2017 February 21).

Disclosure of interest None declared

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