Article Text

Download PDFPDF

Short-term and low-dose IL-2 therapy restores the Th17/Treg balance in the peripheral blood of patients with primary Sjögren’s syndrome
  1. Miao Miao1,
  2. Zhenye Hao1,
  3. Yingying Guo1,
  4. Xiaoying Zhang2,
  5. Shengxiao Zhang1,
  6. Jing Luo2,
  7. Xiangcong Zhao2,
  8. Chen Zhang2,
  9. Xiaoqing Liu1,
  10. Xiaoyan Wu2,
  11. Dan Xu1,
  12. Jinfang Zhao3,
  13. Xuechun Lu4,
  14. Chong Gao5,
  15. Xiaofeng Li2
  1. 1 The Second Hospital of Shanxi Medical University, Taiyuan, China
  2. 2 Department of Rheumatology, The Second Hospital of Shanxi Medical University, Taiyuan, China
  3. 3 Department of Medical Statistics, Shanxi Medical University, Taiyuan, China
  4. 4 Department of Geriatric Hematology, Chinese PLA General Hospital, Beijing, China
  5. 5 Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
  1. Correspondence to Professor Xiaofeng Li, Department of Rheumatology, The Second Hospital of Shanxi Medical University, Taiyuan, China; lxf_9859{at}

Statistics from

Based on evidence that low-dose interleukin-2 (IL-2) increases CD4+CD25+FOXP3+ Treg (CD4Treg) cells in patients with graft-versus-host disease, induction of autoimmune tolerance has been proposed as a treatment.1 However, evidence-based guidelines for the management of primary Sjögren’s syndrome (pSS) are lacking.2 Using a modified method of flow cytometry, we aimed to revaluate the exact levels of Th17 and CD4Treg cells in the peripheral blood (PB) of patients with pSS and explore the effects of short-term and low-dose IL-2.3 4

A total of 190 patients with pSS (169, treated with immunosuppressants; 21, new-onset (sampled as treated or new pSS patients below)) consented at enrolment to donate PB samples for comprehensive immunophenotyping (see online supplementary table S1 and figure S1). In the study, BD Trucount tubes were used to determine the absolute counts of total CD4+ T cells in the PB, and then, the absolute number of CD4+ T subsets, such as CD4Treg cells, were calculated. Detailed methods and statistical analysis is in online version (see online supplementary text). 

Supplementary file 11

Among the CD4+ T subsets in the PB of the healthy control group, Th1 cells had the highest absolute number, and CD4Treg cells were sixfold more abundant than Th17 cells (see online supplementary table S3 and figure 1A–D). Both treated and new pSS patients had significantly fewer absolute CD4Treg cells in their PB (p<0.01) than healthy patients (see figure 1C). CD4Treg cells were the only cell subset with a significant difference in absolute numbers. Notably, neither the absolute number nor proportion of Th17 cells was increased (figure 1A,B). In addition, no significant changes were found in CD4 +CD25+T cells or the percentage of CD4Treg cells (figure 1F,G).5

Supplementary file 3

Figure 1

Characteristics of the absolute numbers and proportions of Th17 cells and CD4Treg cells in the PB of patients with pSS. Lymphocytes in the PB of the healthy control (n=30), treated pSS (n=169) and new pSS (n=21) groups were assessed by flow cytometry. (A and B) Neither the absolute number nor proportion of Th17 cells was altered significantly. (C and D) The absolute number but not the proportion of CD4Treg cells was significantly decreased. (E) Reduction of CD4Treg cells led to an imbalance between Th17 cells and Treg cells (increased ratio of Th17/CD4Tregs). (F and G) Comparison of the absolute number and percentage of CD4Treg cells and CD4 +CD25+T cells among these three groups. All data below are presented as medians (Q1, Q3). Statistical analyses were performed using the independent-samples Kruskal-Wallis test and one-way analysis of variance. *P<0.05; **P<0.01; ***P<0.001 vs healthy control group.

When all patients with pSS were divided into low (0≤ EULAR Sjögren’s syndrome disease activity index (ESSDAI)<5), moderate (5≤ESSDAI≤14) and high (ESSDAI >14) disease activity groups (see online supplementary figure S2), the smallest absolute number of CD4Treg cells in PB were identified in patients with high disease activity (figure 2B). However, no significant difference in levels of Th17 cell was observed among the groups (figure 2A,C).

Supplementary file 8

Figure 2

Relationship between the CD4 +T cell subsets and disease activity and the impact of interleukin (IL) 2 on these cells. (A–C) Correlation of the levels of Th17 cells and CD4Treg cells and the ratio of Th17/Treg cells with disease activity. All patients were divided into low (0≤ESSDAI<5, n=110), medium (5≤ESSDAI≤14, n=67) or high (ESSDAI >14, n=13) disease activity groups. (D–F) After receiving treatment with a short-term low-dose IL-2 (5.0*105 international units (IU) for 5 days) (n=82), CD4Treg cells were amplified more than Th17 cells, and the ratio of Th17/CD4Treg cells was restored. Data are presented as medians (Q1, Q3). Statistical analyses were performed using the independent-samples Kruskal-Wallis test and one-way analysis of variance. *P<0.05; **P<0.01; ***P<0.001.

After low-dose IL-2 treatment, all CD4 +T cells were increased (see online supplementary figure S3), but CD4Treg cells were greatly amplified (fourfold increase) (see online supplementary figure S5, figure 2E). Although the absolute number of Th17 cells also increased, the ratio of Th17/Treg decreased to normal levels (figure 2D,F). Although no obvious improvement in disease activity was found in IL-2 group compared with non-IL-2 group during short-term observation (supplementary figure S4), we observed significant reductions in glucocorticoid and disease-modifying antirheumatic drugs (DMARDS) usage (unpublished data) during long-term observation. No obviously abnormal indicators of laboratory examination were observed during treatment, except for a few cases of rashes around the injection sites and influenza-like symptoms (see online supplementary tables S5–S7). It is possible that low-dose IL-2 can induce antigen-specific CD4+ Treg cells during the treatment process.1 6

Supplementary file 4

Supplementary file 9

Supplementary file 10

In conclusion, our study demonstrates that the absolute number of circulating CD4Treg cells in patients with pSS is significantly lower than that in healthy patients; however, the absolute number and proportion of Th17 cells are not significantly different. Notably, low-dose IL-2 treatment restores CD4Treg cells and the ratio of Th17/Treg which are helpful for controlling disease activity.7–10 Our findings strengthen the concept of immunoregulation but not immunosuppression in the treatment of pSS.1

Supplementary file 1

Supplementary file 2

Supplementary file 5

Supplementary file 6

Supplementary file 7


Ruijie Wu collected the information of outpatients. Qi Wu finished procedures of sampling preparation.



  • Handling editor Josef S Smolen

  • Contributors All of the authors were involved in the drafting of the article or critical revision of the important intellectual content, and all of the authors approved the final version to be published.

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval Ethics Committee of the Second Hospital of Shanxi Medical University.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement The data that support the findings of this study are available from the corresponding.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.