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Abstract
Background Heparan sulfate proteoglycan syndecan-4 plays an important role in inflammation. However, the role of syndecan-4 in rheumatoid arthritis (RA) has not yet been elucidated.
Objectives To detect serum level of syndecan-4 in RA patients and investigate its correlation with RA clinical parameters.
Methods The concentration of serum syndecan-4 was assayed by enzyme-linked immunosorbent assay (ELISA). 43 patients' serum samples from our RA cohort study between 2014 and 2016, and 20 age- and gender-matched osteoarthritis (OA) patients' serum samples were collected and analyzed. Compared the serum syndecan-4 levels in RA patients with DAS28≥3.2 and DAS28<3.2 by Wilcoxon signed rank test. The relationships between serum syndecan-4 levels and RA clinical parameters (DAS28, rheumatoid factor (RF), erythrocyte sedimentation rate, C-reactive protein, etc.) were analyzed.
Results Baseline serum syndecan-4 levels of RA patients were significantly higher than the matched OA patients (1101.56 pg/mL vs 281.41 pg/mL, p<0.001). In RA patients who had sera both at the point of DAS28≥3.2 and DAS28<3.2 (n=13), we found that the former syndecan-4 levels were higher than the latter (1666.22 pg/mL vs 1378.34 pg/mL, p=0.65). The levels of serum syndecan-4 and RF were significantly and positively correlated in RA patients (r=0.696, p=0.008). Furthermore, there is a tendency that serum syndecan-4 levels were higher in the RF-positive (n=31) than in the RF-negative (n=12) RA patients (1344.43 pg/mL vs 971.27 pg/mL, p=0.078).
Conclusions Compared with age- and gender- matched OA patients, serum syndecan-4 concentration is significantly higher in RA patients. Serum syndecan-4 level is positively correlated with RF. Syndecan-4 may play an important role in thes pathogenesis of RA. Further investigation is required to study the mechanism of syndecan-4 in RA.
References
Zhao J, Guo J, Wang L, et al. The role of a proliferation-inducing ligand (APRIL) in the pathogenesis of rheumatoid arthritis. Scand J Rheumatol 2014;43(6):462–9.
Patterson AM, Cartwright A, David G, et al. Differential expression of syndecans and glypicans in chronically inflamed synovium. Ann Rheum Dis 2008 May;67(5):592–601.
Endo T, Ito K, Morimoto J, et al. Syndecan 4 Regulation of the Development of Autoimmune Arthritis in Mice by Modulating B Cell Migration and Germinal Center Formation. Arthritis Rheumatol 2015 Sep;67(9):2512–22.
References
Acknowledgements We are indebted to all the patients who kindly participated in this study. This study was partly supported by Peking University Clinical Research Institute.
Disclosure of Interest None declared