Objectives Smoking has been connected to citrullination of antigens and formation of anti-citrullinated peptide antibodies (ACPAs) in rheumatoid arthritis (RA). Since smoking can modify proteins by carbamylation (formation of homocitrulline), this study was conducted to investigate these effects on vimentin in animal models and RA.
Methods The efficiency of enzymatic carbamylation of vimentin was characterised. B-cell response was investigated after immunisation of rabbits with different vimentin isoforms. Effects of tobacco smoke exposure on carbamylation of vimentin and formation of autoantibodies were analysed in mice. The antibody responses against isoforms of vimentin were characterised with respect to disease duration and smoking status of patients with RA.
Results Enzymatic carbamylation of vimentin was efficiently achieved. Subsequent citrullination of vimentin was not disturbed by homocitrullination. Sera from rabbits immunised with carbamylated vimentin (carbVim), in addition to carbVim also recognised human IgG-Fc showing rheumatoid factor-like reactivity. Smoke-exposed mice contained detectable amounts of carbVim and developed a broad immune response against carbamylated antigens. Although the prevalence of anti-carbamylated antibodies in smokers and non-smokers was similar, the titres of carbamylated antibodies were significantly increased in sera of smoking compared with non-smoking RA. CarbVim antibodies were observed independently of ACPAs in early phases of disease and double-positive patients for anti-mutated citrullinated vimentin (MCV) and anti-carbVim antibodies showed an extended epitope recognition pattern towards MCV.
Conclusions Carbamylation of vimentin is inducible by cigarette smoke exposure. The polyclonal immune response against modified antigens in patients with RA is not exclusively citrulline-specific and carbamylation of antigens could be involved in the pathogenesis of disease.
Trial registration number ISRCTN36745608; EudraCT Number: 2006-003146-41.
- Rheumatoid Arthritis
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Rheumatoid arthritis (RA) is an autoimmune disease in genetically prone individuals where multiple environmental factors can induce the inflammatory process.1 Interestingly, the specific autoimmune response is directed against a number of different antigens modified by post-translational modifications (PTMs).2 In this context, enzymatic deimination of peptide-bound arginine to citrulline by peptidylarginine deiminases (PADs) is considered as a key mechanism.3 Studies in different cohorts of RA have revealed that tobacco exposure increases the risk of anti-citrullinated protein/peptide antibodies (ACPAs) formation, especially in carriers of the shared epitope.1 ,4
Another PTM that has been connected to smoking is carbamylation of lysine, creating a homologous citrulline structure that is extended by a single carbon residual, also known as homocitrulline.5 Various carbamylations are formed by the interaction of isocyanate (HNCO) with α-amino and ε-amino groups of proteins, among them, α-carbamylation, when α-amino groups of amino acids are involved, and ε-carbamylation, which is formed by the interaction of isocyanate with the ε-amino group of lysine.6
In humans, isocyanate is formed by the decomposition of urea into ammonia and cyanate, which is transformed to isocyanate. Thus, uraemia secondary to renal diseases is a pathological state that leads to the formation of isocyanate.7
The second key reactant for isocyanate formation is thiocyanate (SCN−), a metabolite of cyanide that is present in cigarette smoke. Thiocyanate increases in urine, serum and saliva with the amount of cigarette smoke.8 Moreover, passive exposure to smoke significantly increased thiocyanate levels when compared with non-exposure.9 Neutrophil myeloperoxidase (MPO) represents a marker of inflammation and is significantly higher in smokers than non-smokers.10 It uses H2O2 to oxidise thiocyanate to cyanate that subsequently promotes protein carbamylation at sites of inflammation.11
Mice immunised with carbamylated proteins (carbP) developed arthritis and a T-cell response against corresponding antigens. Additionally, antibodies against carbP but not ACPAs were detectable in mice with collagen-induced arthritis.12 ,13 It was also demonstrated that sera from patients with RA contain different antibody specificities recognising either citrulline-containing or homocitrulline-containing antigens,14 ,15 and that anti-carbP as well as ACPAs are detectable even before the onset of symptoms of RA.16 These findings suggest that in addition to citrullination, carbamylation represents another crucial process in the pathogenesis of RA.17
Accumulating evidence suggests an involvement of modified vimentin and anti-modified vimentin antibodies in the pathogenesis of RA.18 ,19 Vimentin is one of the most widely expressed mammalian intermediate filament proteins. The vimentin network that extends from the nucleus to the plasma membrane is believed to act as a scaffold, providing cellular mechanostructural support and thereby maintaining cell and tissue integrity.20 Therefore, we hypothesised that vimentin could serve as a substrate for carbamylation from smoking ingredients and investigated the autoantibody response against different isoforms of vimentin in patients with RA.
Material and methods
Modification of antigens
Non-enzymatic carbamylation of vimentin was achieved by incubation of vimentin with potassium cyanate (KOCN). Incubation conditions and quantification of carbamylation were performed as recently described.21 Enzymatic carbamylation was achieved by incubation of vimentin with MPO (Arotec Diagnostics, New Zealand), according to the previously described procedures.22 As we described recently,21 incubation of recombinant vimentin with KOCN achieved approximately 80% carbamylation.
Animals, immunisation and antibodies
Chicken and rabbit antibodies directed against citrullinated vimentin (citrVim) or carbamylated vimentin (carbVim) were produced by Davids Biotechnology (Regensburg, Germany). New Zealand white rabbits were immunised subcutaneously with 30 µg of peptide emulsified in adjuvant addavax, an MF59-like nanoemulsion; the injections were performed every 14 days for five times. Rabbit polyclonal IgG anti-vimentin antibodies (H-84, Santa Cruz) were employed as reference. To isolate specific antibodies, a previously described procedure for affinity purification was used23 (see online supplementary methods).
Cell fractions as source for modification of antigens
To clarify whether a preferential carbamylation of certain proteins occurs, HeLa cell extracts were used for cell fractionation. Modified and untreated fractions were used to identify specificity of different sera (see online supplementary methods).
For mouse experiments, a whole-body smoking chamber (Teague TE-10) was used as described.24 Mice (C57BL/6) were exposed to tobacco smoke for 6 hours over 5 days weekly for 3 weeks. Control mice breathed filtered air.
Serum samples of the respective mice were investigated for soluble, modified vimentin by using albumin/immunoglobulin depletion columns. Subsequently, the throughput was depleted with trichloroacetic acid (TCA) and separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Detection of modified vimentin was performed with an antibody specific for carbVim. After washing of the separation columns, the eluate from two mice served as controls.
To detect antibodies with different recognition pattern, modified and untreated HeLa cell fractions together with aliquots of unmodified vimentin, citrVim and carbVim were separated by SDS-PAGE and used for immunoblotting with the indicated sera.
ELISA was used to investigate the concentration of anti-carbVim in sera of smoking and control mice.
Immunoblotting using sera from patients with RA
Vimentin, citrVim and carbVim were used to detect the presence of antibodies in sera of patients with RA by immunoblotting.
Detection of rheumatoid factor IgM and anti- mutated citrullinated vimentin IgG antibodies by ELISA
Both antibodies were measured using commercially available ELISA kits (Orgentec Diagnostika, Germany).
Modification specificity of RA autoantibodies and epitope pattern analysis
The predominant vimentin epitope NH2-GGVYATRSSAVR-OH was used to synthesise modified vimentin peptide, citrVim P18 or homocitrulline (carbVim) HC52 (see online supplementary table S1). Both post-translationally modified vimentin peptides were used in ELISA as described,21 ,25 Carbamylated vimentin protein was also used in ELISA as described.21 Additionally, the human full-length vimentin was used to synthesise overlapping 17mer peptides of vimentin resulting in 91 peptides with the general formula ‘Biotin-SGSG-PEPTIDE-Amide’.23
Sera from patients with RA fulfilling the 2010 American College of Rheumatology (ACR)/European League Against Rheumatism criteria (early disease with duration <12 months n=22 and established disease n=28) in addition to 10 healthy donors (HDs) were used for immunoblot experiments and for the analysis of the epitope recognition pattern of patient subgroups regarding the presence of the different autoantibodies. All established patients with RA were treated with conventional disease-modifying antirheumatic drugs (cDMARDs) and four received biological DMARDs (three etanercept and one adalimumab) in combination with cDMARDs. Only two patients with early RA received cDMARDs. A total of 41 patients (15 with Sjögren's syndrome and 26 with systemic lupus erythematosus) in addition to 39 HDs served as control group to calculate sensitivity and specificity of vimentin peptides. All samples were obtained from patients at the Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin, Berlin, Germany, after approval by the local ethics committee.
For ELISA, all patients were recruited from the double-blind, randomised controlled trial HIT HARD.26 All patients (n=172) with recent onset (≤1 year) RA were diagnosed according to the revised 1987 ACR criteria for the classification of RA. Depending on the smoking status, patients were classified as current smokers (n=42), non-smokers (n=68) or ex-smokers (n=34). At week 24, 118 responders and 12 non-responders were identified using the disease activity score 28 erythrocyte sedimentation rate (DAS28ESR) at baseline and week 24 and defined as an improvement of ≥1.2.
GraphPad Prism (V.4.0) and SPSS (V.19.0) software were used for statistics. χ2 test was used for association analyses between smoking and seropositivity of subtype IgG of citrullinated, carbamylated and mutated citrullinated vimentin (MCV) antibodies. ORs were calculated. The paired sample sign test was used to evaluate the differences of antibody titres from baseline to week 24 and week 48. Mann-Whitney U test was used to compare antibody titres between different groups. Correlation analyses were performed using Spearman's rank correlation test (rs). p Values <0.05 were considered significant.
Kinetics of vimentin modification and specificity of antibody responses
To test a possible link between inflammation and tobacco smoking, we used the MPO/H2O2/SCN− system. The amount and quality of homocitrullination of MPO-exposed vimentin was confirmed by immunoblotting using the specific anti-carbVim antibodies (anti-carbVim IgY).
As shown in figure 1A, the anti-carbVim IgY antibodies were specific for carbVim and detected the modified antigen when the reaction lasted for at least 30 min. Anti-vimentin antibodies (H-84) verified the modification of lysine residues by showing a loss of reactivity due to a change of antigenic epitopes after 30 min of antigen treatment. These results indicate that a peroxidase-supported modification system can serve as an alternative tool for homocitrullination. For comparison, detection of citrVim is shown in figure 1B by using anti-vimentin antibodies and affinity-purified human ACPAs. This modification is very efficient and the sequential enzymatic reaction occurs faster than homocitrullination of vimentin in <10 min.
To monitor if carbamylation antagonises arginine deamination (citrullination) or vice versa, both reactions were accomplished sequentially. The respective results are shown in figure 1C demonstrating that the capacity of PAD to deiminate all arginine residues of vimentin is not influenced by the chemical carbamylation, which indicates that in vivo both processes could take place in parallel or in sequential manner.
Different immunogenicity of vimentin isoforms
Rabbits were immunised with vimentin as well as with the citrulline-containing and homocitrulline-containing isoforms and tested for cross-reactivity to carbVim and human IgG-Fc in immunoblots. Immunisation with carbVim induced antibody reactivities against both carbVim and human IgG-Fc, showing rheumatoid factor (RF)-like reactivity. In contrast, rabbits immunised with vimentin or MCV recognised neither carbVim nor human IgG-Fc (figure 2A). Using ELISA, rabbits immunised with citrVim or carbVim were investigated for the presence of cross-reactivity to vimentin, carbVim, citrVim and human IgG-Fc. Sera from rabbits immunised with either citrVim or carbVim showed strong antibody positive bindings specifically to their corresponding induced antigens. In addition to the presence of weak cross-reactivity in both cases, anti-human IgG-Fc reactivity was only observed in homocitrulline-immunised rabbits (figure 2B).
Tobacco exposure to mice induced carbamylation of antigens and antibody response
Sera of smoking mice contained increased amounts of carbVim in comparison with control animals (figure 3A). Smoking mice also generated antibodies with different specificities against carbPs (figure 3B). Additionally, the concentration of anti-carbVim antibodies was significantly increased in sera of smoke-exposed mice compared with controls (figure 3C). However, during an observation period of 3 weeks, no manifestation of an inflammatory joint disease could be observed.
Immunoblotting and epitope recognition patterns of sera from patients with RA
To clarify whether antibodies against carbVim are detectable in patients with early RA and whether they form an own class distinguishable from ACPAs, the respective isoforms were investigated by immunoblotting and ELISA. Immunoblot showed that patients with RA in all stages of disease expressed reactivities against either citrVim, carbVim or both isoforms of vimentin (see online supplementary figure S1).
To characterise the epitope recognition pattern of patient subgroups with respect to the occurrence of the different autoantibodies, overlapping citrullinated peptides resembling the sequence of vimentin were used. Sera from ACPA and/or anti-homocitrulline-positive patients in comparison with HDs (n=10 per group) were investigated using ELISA. An extended recognition pattern was characteristic for patients with both antibody subtypes (anti-carbVim and MCV antibodies, see online supplementary figure S2C), in comparison with a more restricted epitope repertoire of sera containing only antibodies against MCV (see online supplementary figure S2A). Of note, patients with positive bindings to carbVim also showed cross-reactivity with linear citrullinated peptides indicating that their specificity to the full length modified antigen is provided by conformational epitopes (see online supplementary figure S2B). HDs showed negative reactivity (see online supplementary figure S2D). All tested sera of patients with RA showed negative reactivity against unmodified peptide of vimentin.
IgG antibody patterns of patients with RA (HIT HARD) against citrullinated (P18) and carbamylated (HC52) peptides of vimentin using ELISA
Sensitivity, specificity, positive and negative predictive values of these antibodies were calculated to evaluate their efficiency in predicting RA development (table 1).
We compared the titre of carbVim antibodies between different patient groups with RA classified according to smoking habit. The titre of anti-carbVim IgG antibodies was significantly higher in smokers compared with non-smokers. Furthermore, both citrVim and MCV IgG antibodies showed significantly increased titres in smokers compared with non-smokers (figure 4). However, analyses of the prevalence of positivity for each antibody in smokers and non-smokers showed no association between smoking and the presence of anti-carbVim IgG (χ2 p=0.229, OR=1.667, 95% CI 0.722 to 3.848). In contrast, the presence of anti-citrullinated peptide IgG (χ2 p=0.018, OR=2.571, 95% CI 1.161 to 5.694) and anti-MCV IgG (χ2 p=0.005, OR=3.096, 95% CI 1.390 to 6.898) was clearly associated with smoking habit.
Analysis of the coincidence of anti-P18 and anti- HC52 IgG antibodies with anti-MCV IgG showed that 55.55% of smokers who showed positivity for anti-MCV IgG were also positive for anti-carbamylated antibodies (χ2 p=0.0003, OR=38.44, 95% CI 2.086 to 708.3); this percentage declined to 48% in non-smokers (χ2 p=0.0008, OR=7.015, 95% CI 2.073 to 23.74) (see online supplementary table S2).
The prevalence of anticarbamylated IgG antibodies in patients with RA was lower (28%) compared with anti-MCV IgG, anti-citrullinated peptide IgG and RF IgM (48.3%, 50% and 46%, respectively). However, the concentration of both anti-carbamylated and anti-citrullinated peptide IgG antibodies correlated well with anti-MCV IgG in all patients (rs=0.72, 0.81, respectively, p<0.0001 for both). This effect was stronger in smokers (rs=0.82, 0.81, respectively, p <0.0001 for both) compared with non-smokers (rs=0.64, 0.76, respectively, p<0.0001 for both). Correlation between anti-MCV IgG and RF IgM in all patients was weak (rs=0.63, p <0.0001) and not restricted to smoking status.
To clarify whether the titre of anti-citrullinated and anti-carbamylated IgG antibodies correlates with disease activity in patients with RA, we classified the patients into treatment responders and non-responders according to DAS28ESR. By comparing the differences from baseline to 24 and 48 weeks after therapy in both groups, the titre reduction did not reflect improved treatment response for all tested antibodies (table 2). However, at 24 and 48 weeks, a significant titre reduction of anti-MCV IgG, RF IgM and to a lesser extent of anti-citrullinated and anti-carbamylated antibodies was observed in responders with the exception of those with constant high disease activity. Due to low numbers of non-responders, no statistical evaluation was possible.
Notably, the therapeutic effect for induction of response and remission was affected by smoking habit; thus, whereas almost 55% of non-smokers achieved remission, only 40% of smokers and 36% of ex-smokers reached the same outcome.
Smoking has been identified as a major risk factor for the induction and perpetuation of RA.27 The current pathogenetic model suggests citrullination of antigens in the lungs and elsewhere as a driving process.28 However, it seems that the significance of other PTMs such as carbamylation has not been recognised sufficiently so far.
In this study, we characterised the extent and the kinetics of carbamylation of vimentin as a well-known autoantigen with close association to the pathogenesis of RA. We provided clear evidence that carbVim and not citrVim is a strong antigen that induces a B-cell response against itself and against human IgG-Fc in an animal model, thus inducing a RF-like activity. Almost the whole spectrum of autoantibodies characteristic of RA was induced. Moreover, in agreement with other studies,29 ,30 we showed that the autoimmune response against carbVim is an early event in the pathogenesis of RA. Additionally, we proved that vimentin, as a major and well-characterised autoantigen in RA, does form its own class of carbamylated antigens that could be strongly recognised by antisera induced only with the corresponding antigen in an animal model. The cross-reactions, which have been seen in carbVim-positive RA sera as well as in antisera of rabbits immunised with carbVim, could occur in ureido group-specific manner. Furthermore, we showed that both carbamylation and citrullination are independent processes that may happen in parallel or in sequential manner.
In a mouse model, we showed that carbamylation of vimentin can be triggered by exposure to tobacco smoke and that this antigen and potentially other modified proteins are able to induce a broad immune reaction against many variants of carbP. Additionally, a significantly increased level of anti-carbamylated antibodies was observed in smoke-exposed mice and smoking patients with RA when compared with filtered air-exposed or non-smoking controls. However, in agreement with other studies,25 ,29 no association between smoking and anti-carbamylated antibody positivity was detected in patients with RA. These findings support the idea that carbamylation in smokers and non-smokers could be induced by additional triggering factors including even passive exposure to tobacco smoke. Nevertheless, smoking induced increased titres of carbamylated antibodies in humans and mouse models.
In our previous study on Cuban patients,21 the prevalence of IgG antibodies against carbamylated peptides of vimentin was higher (60% and almost 80% in early and established RA, respectively) than in the present study (28%). It has been shown that the combination of major histocompatibility complex, class II, DR beta 1 (HLA-DRB1)-shared epitope alleles and smoking is associated with RA susceptibility31 and different genetic associations of autoantibody-positive disease subgroups was identified in relation to the presence of DRB1*04.32 These findings suggest that the discrepancy in the frequencies of autoantibodies between different populations could be attributed to the genetic heterogeneity, as well as different environmental and behavioural factors.
Notably, the epitope recognition patterns and the coincidences of anti-carbamylated with anti-MCV antibodies suggest a strong association between these antibodies in RA. This is in agreement with a recently described association of anti-carbP and anti-citrVim that could not be explained by cross-reactivity.33
Of note, a treatment-related reduction of antibody titres was less obvious for anti-carbamylated antibodies compared with all other tested antibodies. This indicates that anti-carbamylated antibodies are produced by a distinct B-cell subset, which appears to be more treatment-resistant.
In conclusion, we demonstrated that the polyclonal immune response against modified antigens in RA is restricted to citrullinated and carbamylated autoantigens, which could be involved in the pathogenesis of disease and response to therapy. Direct or indirect exposure to tobacco smoke could have an effect by inducing carbamylation, which could trigger a pathogenic B-cell response.
The authors thank Tanja Braun for her support in clinical data collection in the randomised controlled trial (RCT) HIT HARD and Annushka Wurdinger for assistance in immunoblotting.
Handling editor Tore K Kvien
KG and GRB have equally contributed to the work.
Contributors CO performed the analysis of the mouse experiments and contributed in writing and reviewing the manuscript; HB advised vimentin carbamylation, animal immunisation, HeLa cell fractionation, Western blot analysis, ELISA kits developing and contributed in writing and reviewing the manuscript; EF designed the study, collected the results and contributed in writing and reviewing the manuscript; GC and SK performed and designed the analysis of the mouse experiments and contributed in reviewing the manuscript; JD collected samples and clinical data of RCT HIT HARD and contributed in reviewing the manuscript; AK developed and performed the analysis of the ELISA kits, immunoblot experiments and contributed in reviewing the manuscript; SG contributed to the study design and in reviewing the manuscript; KG performed and designed the analysis of ELISA experiments, collected clinical data and contributed in writing and reviewing the manuscript and GRB contributed to the study design and in writing and reviewing the manuscript.
Funding The study was supported by the FP7 HEALTH programme under the grant agreement FP7-HEALTHF2-2012-305549, HIT HARD study (German Federal Ministry of Education and Research (BMBF, grant number: 01KG0602) and EU IMI grant BeTheCure, contract no 115142-2; ArthroMark of the German Federal Ministry of Education and Research (BMBF, grant number: 01EC1401A).
Competing interests HB is an employee of Orgentec Diagnostika GmbH, Mainz, Germany; test kits were kindly provided by Orgentec.
Patient consent Obtained.
Ethics approval Charité-Universitätsmedizin Berlin, Germany.
Provenance and peer review Not commissioned; externally peer reviewed.
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