Background Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of multiple autoantibodies by hyperactive B cells. B-1 cells distinguished from conventional B cell, perform a protective role in defending against microbial pathogens by secreting natural antibodies. Recently, human B-1 cells identified phenotypically according to the characterization of murine B-1 cells, were reported to be increased in peripheral blood of SLE patients.
Objectives The aim of this study is to investigate the frequency of B-1 cells and its correlation with disease activity in SLE.
Methods Peripheral blood mononuclear cells were isolated from 21 SLE patients and 16 age/sex matched healthy controls. Frequencies of B-1 cells were analyzed by multicolor flow cytometry using antibodies against CD3, CD20, CD27, CD43, and CD70. Phenotype of human B-1 cells was identified by CD3-CD20+CD27+CD43+CD70-. Correlation of B-1 cell frequency with the disease activity of SLE was analyzed according to anti-dsDNA antibody, complement level, renal involvement, platelets count, white blood cell count, erythrocyte sedimentation rate (ESR), and SLE disease activity index (SLEDAI) score.
Results Absolute B cell count was significantly decreased in SLE patients compared to healthy controls (mean ± SE: 29813±8096 cells/ml vs. 62593±10465 cells/ml, p=0.002). Frequencies of B-1 cell in total B cells were significantly increased in SLE patients compared to healthy controls (1.86±0.33 vs. 0.79±0.10, p=0.025). SLE patients with anti-dsDNA antibody tended to have higher frequency of B-1 cells than patients without anti-dsDNA antibody (1.92±0.39 vs. 1.77±0.60). However there was no correlation between complement level, renal involvement, platelets count, white blood cell count, ESR, or SLEDAI score and B-1 cell frequency.
Conclusions The frequencies of B-1 cell were significantly higher in SLE compared to healthy controls. But there was no correlation between disease activity and B-1 cell frequency.
Griffin DO, Holodick NE, and Rothstein TL. Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+ CD27+ CD43+ CD70-. J. Exp. Med 2011;208:67-80.
Griffin DO, and Rothstein TL. Human B1 cell frequency: isolation and analysis of human B1 cells. Front. Immunol. 2012;3:122.
Tørring C, Petersen CC, Bjerg L, et al. The B1-cell subpopulation is diminished in patients with relapsing-remitting multiple sclerosis. J Neuroimmunol. 2013;262:92-99.
Disclosure of Interest None declared
Statistics from Altmetric.com
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.