Objectives Behçet’s disease (BD) as systemic vasculitis of unknown etiology is associated with HLA-B*51 in different European and Asian populations. HLA-A*26 was claimed as additional BD susceptibility marker in Japanese and Greek patients independent of HLA-B*51. This study was performed to test for HLA-A associations in German and Turkish BD populations. HLA-B*51 negative BD patients were compared with respective control populations.
Methods 62 German and 44 Turkish patients lacking HLA-B*51 were analyzed in comparison to healthy German (N=1500) and Turks (N=77) negative for B*51. HLA-A/B genotypes were determined by SSOP (Luminex Corp.) Fisher’s exact test and Odds ratio (OR) were used for statistical evaluation.
Results HLA-A*26 was significantly more frequent in German patients (14.52% patients vs. 5.9% healthy Germans, chi-square =6,07, OR =2,69, p<0.0137). When individuals carrying a second Bw4+ HLA-B locus allele were excluded from this analysis, HLA-A*26 association with BD was no longer observed. Frequency of HLA-Bw4+ (HLA-A and B alleles) alleles, however, was significantly different in German B*51 negative BD patients versus controls (patients vs. controls: 72.6% vs. 54.5%, chi-square =7,18, OR =2,21, p<0.0058).In addition a significant decrease in the frequency of HLA-A*03 was observed in German BD patients (patients vs. controls: 14.5% vs. 30.1%, chi-square =6,20, OR =0,39, p<0.0127).No significant association of any of the tested HLA-A alleles nor of the HLA-Bw4 determinant in B*51 negative Turkish patients was seen.
Conclusions Although B*51 negative German BD patients showed an association with HLA-A*26 as in Japanese and Greek, this appeared to reflect a higher frequency of Bw4+alleles inherited in linkage. Thus, stronger association with HLA-Bw4 in the B*51 negative Germans may indicate relevance in BD pathogenesis. A protective factor may be inherited in association with HLA-A*03 in Germans. No BD association to HLA-A alleles was detected in Turks, possibly due to the low numbers of patients analyzed.
Disclosure of Interest A. Kuranov Grant/Research support from: DAAD, F. Riewerts: None Declared, I. Kötter: None Declared, J. Gamper-Tsigaras: None Declared, I. Steiert: None Declared, C. Müller: None Declared
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