Background Pre-eclampsia affects 2-7% of all pregnancies (1), is a leading cause of maternal morbidity and mortality and increases perinatal mortality five-fold (2). Women with Systemic Lupus Erythematosus (SLE) have a particularly high risk of developing pre-eclampsia (3) and patients with specific organ involvement (i.e. lupus nephritis) are at higher risk of developing pre-eclampsia earlier in their pregnancy (4).
Objectives This study aims to identify human pregnancy biomarkers in the urinary proteome to improve the diagnosis and/or prediction of pre-eclampsia in patients with SLE.
Methods Second trimester urine samples were collected from 3 women with SLE and lupus nephritis who subsequently developed pre-eclampsia, 5 SLE gestation-matched patients who did not develop pre-eclampsia and 6 healthy controls. Samples were prepared following our optimized workflow using in-gel trypsin digestion followed by LC-MS/MS. Spectral abundance of differentially expressed proteins was analysed using minimal stringency settings in Scaffold software.
Results Our preliminary data suggest that pre-eclampsia results in a distinctive urinary proteome in women with lupus nephritis, but also in controls compared to women without pre-eclampsia (Figure 1a and b). One hundred and sixteen proteins were identified; of these, 6 proteins were found to be present only in the SLE pre-eclampsia urine samples (arrow). Furthermore, two proteins were found to be present in SLE pre-eclampsia (arrow), but showed a difference in SLE non-pre-eclampsia urinary samples and were absent in the samples from women with SLE without pre-eclampsia (circled in black) (Figure 1b).
Conclusions Previous work in this group involving pre-eclamptic samples has used targeted mass spectrometry for relative quantitation in a validation sample set. Our study shows differentially expressed proteins, representing potential biomarker candidates of pre-eclampsia in patients with SLE/APS and hence warrant further investigation.
Sibai B et al., Lancet 2005;365:785-99.
Roberts J.M.; Placenta 2002;23:359-72.
Salmon JE et al., PLoS Med. 2011;8:e1001013.
Bramham K et al., J Rheumatol 2011 Jun 1.
Disclosure of Interest K. Schreiber Grant/Research support from: ESF - Biolupus, K. Bramham: None Declared, H. Mistry: None Declared, O. Barrutia-Ateka: None Declared, S. Lynham: None Declared, A. Weston: None Declared, M. Ward: None Declared, L. M. Bertolaccini: None Declared, L. C. Chappell: None Declared, M. Khamashta: None Declared
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