Confocal microscopy of immunofl uorescence for α-SMA in cultured normal and NSF dermal fibroblasts. NSF dermal fibroblasts display prominent α-SMA expression and intracellular filament organisation. α-SMA fl uorescence is in red. 4′,6-Diamidino-2-phenylindole staining of all nuclei appears in blue/purple. NSF, nephrogenic systemic fibrosis. See page 2022.