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IL1 and TNF gene polymorphisms in patients with juvenile idiopathic arthritis treated with TNF inhibitors
  1. Rolando Cimaz1,
  2. Marie-Angélique Cazalis1,
  3. Charlotte Reynaud1,
  4. Valeria Gerloni2,
  5. Francesco Zulian3,
  6. Martina Biggioggero2,
  7. Giorgia Martini3,
  8. Irene Pontikaki2,
  9. Flavio Fantini2,
  10. Bruno Mougin1,
  11. Pierre Miossec1
  1. 1Unité Mixte Hospices Civils de Lyon-BioMérieux, Lyon, France
  2. 2Pediatric Rheumatology, Ospedale Gaetano Pini and University of Milano, Milan, Italy
  3. 3Department of Pediatrics, University of Padova, Padova, Italy
  1. Correspondence to:
    Dr R Cimaz
    Universitè Claude Bernard Lyon 1, Unité Mixte Hospices Civils de Lyon-BioMérieux, Lyon, France; roland.cimaz{at}


Objective: To investigate the genetic contribution of cytokine gene polymorphisms (interleukin 1 (IL1) and tumour necrosis factor α (TNFα)) on disease phenotype and on response to TNF-blocking agents in a population of patients with juvenile idiopathic arthritis (JIA).

Methods: A cohort of 107 consecutive patients with JIA who were receiving treatment with anti-TNF agents was enrolled in this study. Analysis of genetic polymorphisms for IL1B +3954, IL1RA +2018, TNFα −238 and TNFα −308 was performed by enzyme-linked oligo sorbent assay, and compared with those obtained from 630 healthy Caucasians and 263 adult patients with rheumatoid arthritis. Relevant demographic, clinical and laboratory data were collected from clinical charts and entered into a customised database, and χ2 analysis was performed to compare cytokine polymorphisms with disease type according to the International League of Associations for Rheumatology criteria, presence of uveitis, rheumatoid factor and anti-nuclear antibody positivity, erosive disease, frequency of adverse effects to anti-TNF and clinical response after 3 months.

Results: The T/T genotype of the IL1B +3954 polymorphism was absent in patients with JIA and present in 5% of controls (p = 0.015). No significant correlation was found between the studied polymorphisms and clinical or laboratory variables considered. Clinical response to TNF inhibitors at 3 months was not associated with the genetic polymorphisms considered.

Conclusion: In our cohort, the absence of the rare IL1B +3954 gene polymorphism was associated with JIA, but without specificity to particular disease phenotypes. The TNF and IL1 gene polymorphism studied did not seem to be associated with response to anti-TNF treatment.

  • IL, interleukin
  • ILAR, International League of Associations for Rheumatology
  • JIA, juvenile idiopathic arthritis
  • RA, rheumatoid arthritis
  • SNP, single nucleotide polymorphism
  • TNFα, tumour necrosis factor α

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  • Published Online First 26 February 2007

  • Funding: This study has been partly funded by a grant from parents’ association “Kourir”.

  • Competing interests: None declared.