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Activation dependent apoptosis of peripheral blood mononuclear cells from patients with rheumatoid arthritis treated with methotrexate
  1. J Swierkot1,
  2. R Miedzybrodzki2,
  3. S Szymaniec2,
  4. J Szechinski1
  1. 1Medical University Wroclaw, Poland
  2. 2Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
  1. Correspondence to:
    Dr J Swierkot
    Medical University Wroclaw, ul. Wisniowa 36 Wroclaw, Poland 53-137;

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Activation-induced apoptosis is a critical mechanism by which the immune system maintains tolerance to self antigens by the clonal deletion of autoreactive T and B cells.1 The involvement of apoptosis in the mechanism of cell death induced by high doses of methotrexate (MTX) was first demonstrated in Chinese hamster ovary cells.2 Fas mediated apoptosis may have a critical role in the regression of synovial hyperplasia in rheumatoid arthritis (RA).3 Cutolo et al demonstrated the influence of MTX on the proliferation and apoptosis of cultured human monocytic myeloid cells (THP-1).4 Genestier et al investigated the effect of low doses of MTX on human peripheral blood lymphocytes and showed that MTX could induce the in vitro apoptosis of mitogen activated lymphocytes CD4+ and CD8+ but not resting T cells. He also demonstrated that a single injection of MTX during low dose treatment in six patients with RA was sufficient to prime lymphocytes …

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